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Capillary electrophoresis for automated on-line monitoring of suspension cultures: Correlating cell density, nutrients and metabolites in near real-time

journal contribution
posted on 2023-05-18, 18:44 authored by Alhusban, AA, Michael BreadmoreMichael Breadmore, Nuri GuvenNuri Guven, Rosanne Guijt
Increasingly stringent demands on the production of biopharmaceuticals demand monitoring of process parameters that impact on their quality. We developed an automated platform for on-line, near real-time monitoring of suspension cultures by integrating microfluidic components for cell counting and filtration with a high-resolution separation technique. This enabled the correlation of the growth of a human lymphocyte cell line with changes in the essential metabolic markers, glucose, glutamine, leucine/isoleucine and lactate, determined by Sequential Injection-Capillary Electrophoresis (SI-CE). Using 8.1 mL of media (41 μL per run), the metabolic status and cell density were recorded every 30 min over 4 days. The presented platform is flexible, simple and automated and allows for fast, robust and sensitive analysis with low sample consumption and high sample throughput. It is compatible with up- and out-scaling, and as such provides a promising new solution to meet the future demands in process monitoring in the biopharmaceutical industry.

History

Publication title

Analytica Chimica Acta

Volume

920

Pagination

94-101

ISSN

0003-2670

Department/School

School of Pharmacy and Pharmacology

Publisher

Elsevier Science Bv

Place of publication

Netherlands

Rights statement

Copyright 2016 Elsevier B.V.

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the chemical sciences

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