eCite Digital Repository

Capillary electrophoresis for automated on-line monitoring of suspension cultures: Correlating cell density, nutrients and metabolites in near real-time

Citation

Alhusban, AA and Breadmore, MC and Gueven, N and Guijt, RM, Capillary electrophoresis for automated on-line monitoring of suspension cultures: Correlating cell density, nutrients and metabolites in near real-time, Analytica Chimica Acta, 920 pp. 94-101. ISSN 0003-2670 (2016) [Refereed Article]

Copyright Statement

Copyright 2016 Elsevier B.V.

DOI: doi:10.1016/j.aca.2016.03.034

Abstract

Increasingly stringent demands on the production of biopharmaceuticals demand monitoring of process parameters that impact on their quality. We developed an automated platform for on-line, near real-time monitoring of suspension cultures by integrating microfluidic components for cell counting and filtration with a high-resolution separation technique. This enabled the correlation of the growth of a human lymphocyte cell line with changes in the essential metabolic markers, glucose, glutamine, leucine/isoleucine and lactate, determined by Sequential Injection-Capillary Electrophoresis (SI-CE). Using 8.1 mL of media (41 μL per run), the metabolic status and cell density were recorded every 30 min over 4 days. The presented platform is flexible, simple and automated and allows for fast, robust and sensitive analysis with low sample consumption and high sample throughput. It is compatible with up- and out-scaling, and as such provides a promising new solution to meet the future demands in process monitoring in the biopharmaceutical industry.

Item Details

Item Type:Refereed Article
Keywords:sequential injection capillary electrophoresis, bioprocess monitoring, online monitoring, bioreactor, capillary electrophoresis, suspension cell culture, lactate, glucose, glutamine
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Alhusban, AA (Mr Ala Alhusban)
Author:Breadmore, MC (Professor Michael Breadmore)
Author:Gueven, N (Dr Nuri Guven)
Author:Guijt, RM (Dr Rosanne Guijt)
ID Code:108290
Year Published:2016
Web of Science® Times Cited:2
Deposited By:Pharmacy
Deposited On:2016-04-18
Last Modified:2017-10-31
Downloads:0

Repository Staff Only: item control page