Increasingly stringent demands on the production of biopharmaceuticals demand monitoring of process parameters that impact on their quality. We developed an automated platform for on-line, near real-time monitoring of suspension cultures by integrating microfluidic components for cell counting and filtration with a high-resolution separation technique. This enabled the correlation of the growth of a human lymphocyte cell line with changes in the essential metabolic markers, glucose, glutamine, leucine/isoleucine and lactate, determined by Sequential Injection-Capillary Electrophoresis (SI-CE). Using 8.1 mL of media (41 μL per run), the metabolic status and cell density were recorded every 30 min over 4 days. The presented platform is flexible, simple and automated and allows for fast, robust and sensitive analysis with low sample consumption and high sample throughput. It is compatible with up- and out-scaling, and as such provides a promising new solution to meet the future demands in process monitoring in the biopharmaceutical industry.

Item Type:	Refereed Article
Keywords:	sequential injection capillary electrophoresis, bioprocess monitoring, online monitoring, bioreactor, capillary electrophoresis, suspension cell culture, lactate, glucose, glutamine
Research Division:	Chemical Sciences
Research Group:	Analytical chemistry
Research Field:	Separation science
Objective Division:	Expanding Knowledge
Objective Group:	Expanding knowledge
Objective Field:	Expanding knowledge in the chemical sciences
UTAS Author:	Alhusban, AA (Mr Ala Alhusban)
UTAS Author:	Breadmore, MC (Professor Michael Breadmore)
UTAS Author:	Gueven, N (Dr Nuri Guven)
UTAS Author:	Guijt, RM (Dr Rosanne Guijt)
ID Code:	108290
Year Published:	2016
Web of Science® Times Cited:	17
Deposited By:	Pharmacy
Deposited On:	2016-04-18
Last Modified:	2017-10-31
Downloads:	0