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Association of bovine fatty acid desaturase 2 gene single-nucleotide polymorphisms with intramuscular fatty acid composition in Japanese Black Steers


Takahashi, H and Hayashi, M and Ushizawa, K and Nishino, K and Haga, Y and Saito, T and Fujimori, Y and Iwama, N and Takeda, H and Komatsu, M and Malau-Aduli, AEO, Association of bovine fatty acid desaturase 2 gene single-nucleotide polymorphisms with intramuscular fatty acid composition in Japanese Black Steers, Open Journal of Animal Sciences, 6, (2) pp. 105-115. ISSN 2161-7597 (2016) [Refereed Article]


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Copyright 2016 the authors and Scientific Research Publishing Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0)

DOI: doi:10.4236/ojas.2016.62013


Beef from Japanese Black cattle (JBK), is popular in Japan and valued for its highly marbled fat content. In JBK, genes affecting oleic acid content in meat have been studied mainly to lower the fat melting point and improve tenderness; however, there has been no direct correlation demonstrated between beef taste and oleic acid. To investigate genes affecting other fatty acids other than oleic acid, polymorphisms of the fatty acid desaturase 2 (FADS2) gene were genotyped and associations with fatty acid profile in JBK beef were investigated. Amplifications of 5-flanking regions, 12 exons, and 3-untranslated regions of the FADS2 gene in three Japanese and five Western cattle breeds via PCR, were amplified, sequenced and SNPs were identified using specific TaqMan genotyping assay. Fatty acid composition of intramuscular adipose tissue of the Trapezius muscle was analyzed in JBK steers. Six of the 15 identified SNPs are novel and have never been registered in any public bovine SNP database. A non-synonymous SNP (rs211580559; C > T; 294 Ala > Val) in exon 7 was examined in order to evaluate its association with fatty acid profiles. The data showed that highly significant association existed between rs211580559 and C18:2 (n-6) composition, and accounted for 22.3% of the variation. There were no significant relationships between rs2115-80559 and the other fatty acids. It was concluded that rs211580559 of the FADS2 gene may be a useful selection marker for reducing unfavorable volatiles generated from linoleic acid in JBK beef during the cooking process.

Item Details

Item Type:Refereed Article
Keywords:Japanese Black Cattle, beef, fatty acid desaturase 2, single-nucleotide polymorphism, fatty acid composition
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Animal production
Research Field:Animal reproduction and breeding
Objective Division:Animal Production and Animal Primary Products
Objective Group:Livestock raising
Objective Field:Beef cattle
UTAS Author:Malau-Aduli, AEO (Associate Professor Aduli Malau-Aduli)
ID Code:108259
Year Published:2016
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2016-04-15
Last Modified:2017-04-24
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