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Capsaicin-induced death of human haematological malignant cell lines is independent of TRPV1 activation

Citation

Omari, SA and Adams, MJ and Kunde, DA and Geraghty, DP, Capsaicin-induced death of human haematological malignant cell lines is independent of TRPV1 activation, Pharmacology, 98, (1-2) pp. 79-86. ISSN 0031-7012 (2016) [Refereed Article]

Copyright Statement

Copyright 2016 Karger AG, Basel

DOI: doi:10.1159/000445437

Abstract

The effect of the plant-derived vanilloid, capsaicin (CAP), on the metabolic activity of THP-1, U266B1 and U937 hematological malignancy cells was determined. CAP reduced metabolic activity in a concentration-dependent manner in the three cell lines. A biphasic effect was observed on THP-1 cells (EC50: IC50 (95% CI) 32.9 (19.9-54.3)/219 (144-246) Ámol/l). U266B1 cells were more resistant to CAP than THP-1 and U937. Metabolic activity was significantly inhibited by CAP in U937 compared to U266B1 cells (IC50: 197 versus 431 Ámol/l, respectively, p < 0.008). Transient receptor potential vanilloid-1 (TRPV1) and CB1 antagonists (SB452533 and AM251, respectively) suppressed the CAP-induced increase in THP-1 cell metabolic activity (p < 0.001). AM251 and SB452533 appeared to act as partial agonists and displayed a synergistic effect with CAP in U937 cells. CAP inhibits the metabolic activity of malignant hematological cells through non-TRPV1-dependent mechanisms.

Item Details

Item Type:Refereed Article
Keywords:vanilloids, platelets, capsaicin, cell metabolic activity, THP-1, U266U937, transient receptor potential vanilloid-11
Research Division:Medical and Health Sciences
Research Group:Pharmacology and Pharmaceutical Sciences
Research Field:Pharmacology and Pharmaceutical Sciences not elsewhere classified
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Blood Disorders
Author:Omari, SA (Dr Sofia Omari)
Author:Adams, MJ (Dr Murray Adams)
Author:Kunde, DA (Dr Dale Kunde)
Author:Geraghty, DP (Professor Dominic Geraghty)
ID Code:107948
Year Published:2016
Deposited By:Health Sciences
Deposited On:2016-04-01
Last Modified:2017-12-21
Downloads:0

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