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Inhibition of platelet aggregation by vanilloid-like agents is not mediated by transient receptor potential vanilloid-1 channels or cannabinoid receptors

Citation

Almaghrabi, S and Geraghty, DP and Ahuja, KDK and Adams, MJ, Inhibition of platelet aggregation by vanilloid-like agents is not mediated by transient receptor potential vanilloid-1 channels or cannabinoid receptors, Clinical and Experimental Pharmacology and Physiology, 43, (6) pp. 606-611. ISSN 1440-1681 (2016) [Refereed Article]

Copyright Statement

Copyright 2016 John Wiley & Sons Australia, Ltd

DOI: doi:10.1111/1440-1681.12569

Abstract

Vanilloid-like agents, including capsaicin, N-arachidonoyldopamine and N-oleoyldopamine inhibit platelet aggregation, however little is known about the precise mechanism(s) of action. The authors have previously shown that blocking of the capsaicin receptor, transient receptor potential vanilloid-1 (TRPV1), does not interfere with capsaicin action during adenosine diphosphate (ADP)-induced aggregation. This research is here extended to investigate the effect of these vanilloidlike- agents on platelet count, and to test whether the effect of these agents is mediated through TRPV1 and/or cannabinoid (CB1 and CB2) receptors in presence of other agonists, including collagen and arachidonic acid. Incubation of platelets with each of the individual vanilloids, or with receptor antagonists of TRPV1 (SB452533), CB1 (AM251) and CB2 (AM630), for up to 2 h did not signicantly affect the platelet count. Similarly, the effect of individual vanilloids on the inhibition of platelet aggregation was not signicantly different in the presence of receptor agonists compared to control, irrespective of the agonist used, suggesting that the inhibitory effect of vanilloids on platelet aggregation is independent of TRPV1, CB1 and CB2 receptors. Further research on the antiplatelet activity of vanilloids should focus on mechanisms other than those associated with vanilloid receptors. Key words: cannabinoid, capsaicin, N-arachidonoyldopamine, N-oleoyldopamine, transient receptor potential vanilloid-1.

Item Details

Item Type:Refereed Article
Keywords:Vanilloids, capsaicin, platelets
Research Division:Medical and Health Sciences
Research Group:Pharmacology and Pharmaceutical Sciences
Research Field:Pharmacology and Pharmaceutical Sciences not elsewhere classified
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Blood Disorders
Author:Almaghrabi, S (Ms Safa Almaghrabi)
Author:Geraghty, DP (Professor Dominic Geraghty)
Author:Ahuja, KDK (Dr Kiran Ahuja)
Author:Adams, MJ (Dr Murray Adams)
ID Code:107945
Year Published:2016
Deposited By:Health Sciences
Deposited On:2016-04-01
Last Modified:2017-04-26
Downloads:0

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