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Laser-mediated microlesions in mouse neocortex to investigate neuronal degeneration and regeneration

Citation

Jackson, J and Canty, AJ and Huang, L and De Paola, V, Laser-mediated microlesions in mouse neocortex to investigate neuronal degeneration and regeneration, Current Protocols in Neuroscience, 73 Article 2.24. ISSN 1934-8584 (2015) [Refereed Article]

Copyright Statement

Copyright 2015 John Wiley & Sons, Inc.

DOI: doi:10.1002/0471142301.ns0224s73

Abstract

In vivo two-photon (2P) imaging enables neural circuitry to be repeatedly visualized in both normal conditions and following trauma. This protocol describes how laser-mediated neuronal microlesions can be created in the cerebral cortex using an ultrafast laser without causing a significant inflammatory reaction or compromising the blood-brain barrier. Furthermore, directives are provided for the acute and chronic in vivo imaging of the lesion site, as well as for post-hoc analysis of the lesion site in fixed tissue, which can be correlated with the live imaging phase.

Item Details

Item Type:Refereed Article
Keywords:microlesion, live imaging, laser ablation, axotomy, 2-photon microscopy, glial scar, axons, synapses, cortex, laser lesion, degeneration, 2 photon imaging, brain injury, trauma
Research Division:Medical and Health Sciences
Research Group:Neurosciences
Research Field:Cellular Nervous System
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Nervous System and Disorders
Author:Canty, AJ (Associate Professor Alison Canty)
ID Code:107740
Year Published:2015
Deposited By:Wicking Dementia Research and Education Centre
Deposited On:2016-03-22
Last Modified:2016-08-24
Downloads:0

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