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Electrical Stimulation Promotes Cardiac Differentiation of Human Induced Pluripotent Stem Cells

Citation

Hernandez, D and Millard, R and Sivakumaran, P and Wong, RCB and Crombie, DE and Hewitt, AW and Liang, H and Hung, SSC and Pebay, A and Shepherd, RK and Dusting, GJ and Lim, SY, Electrical Stimulation Promotes Cardiac Differentiation of Human Induced Pluripotent Stem Cells, Stem Cells International Article 1718041. ISSN 1687-966X (2016) [Refereed Article]


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Copyright Statement

Copyright 2016 Damian Hernandez et al. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.1155/2016/1718041

Abstract

Background. Human induced pluripotent stem cells (iPSCs) are an attractive source of cardiomyocytes for cardiac repair and regeneration. In this study, we aim to determine whether acute electrical stimulation of human iPSCs can promote their differentiation to cardiomyocytes.

Methods. Human iPSCs were differentiated to cardiac cells by forming embryoid bodies (EBs) for 5 days. EBs were then subjected to brief electrical stimulation and plated down for 14 days.

Results. In iPS(Foreskin)-2 cell line, brief electrical stimulation at 65 mV/mm or 200 mV/mm for 5 min significantly increased the percentage of beating EBs present by day 14 after plating. Acute electrical stimulation also significantly increased the cardiac gene expression of ACTC1, TNNT2, MYH7, and MYL7. However, the cardiogenic effect of electrical stimulation was not reproducible in another iPS cell line, CERA007c6. Beating EBs from control and electrically stimulated groups expressed various cardiac-specific transcription factors and contractile muscle markers. Beating EBs were also shown to cycle calcium and were responsive to the chronotropic agents, isoproterenol and carbamylcholine, in a concentration-dependent manner.

Conclusions. Our results demonstrate that brief electrical stimulation can promote cardiac differentiation of human iPS cells. The cardiogenic effect of brief electrical stimulation is dependent on the cell line used.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Ophthalmology and Optometry
Research Field:Ophthalmology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Hearing, Vision, Speech and Their Disorders
Author:Hewitt, AW (Dr Alex Hewitt)
ID Code:107719
Year Published:2016
Web of Science® Times Cited:4
Deposited By:Menzies Institute for Medical Research
Deposited On:2016-03-22
Last Modified:2017-11-03
Downloads:58 View Download Statistics

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