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Rutin, a flavonoid with antioxidant activity, improves plant salinity tolerance by regulating K+ retention and Na+ exclusion from leaf mesophyll in quinoa and broad beans
Citation
Ismail, H and Maksimovic, JD and Maksimovic, V and Shabala, L and Zivanovic, BD and Tian, Y and Jacobsen, S-E and Shabala, S, Rutin, a flavonoid with antioxidant activity, improves plant salinity tolerance by regulating K+ retention and Na+ exclusion from leaf mesophyll in quinoa and broad beans, Functional Plant Biology, 43, (1) pp. 75-86. ISSN 1445-4408 (2016) [Refereed Article]
Copyright Statement
Copyright 2016 CSIRO
DOI: doi:10.1071/FP15312
Abstract
The causal relationship between salinity and oxidative stress tolerance is well established, but specific downstream targets and the role of specific antioxidant compounds in controlling cellular ionic homeostasis remains elusive. In this work, we have compared antioxidant profiles of leaves of two quinoa genotypes contrasting in their salt tolerance, with the aim of understanding the role of enzymatic and non-enzymatic antioxidants in salinity stress tolerance. Only changes in superoxide dismutase activity were correlated with plant adaptive responses to salinity. Proline accumulation played no major role in either osmotic adjustment or in the tissue tolerance mechanism. Among other non-enzymatic antioxidants, rutin levels were increased by over 25 fold in quinoa leaves. Exogenous application of rutin to glycophyte bean leaves improved tissue tolerance and reduced detrimental effects of salinity on leaf photochemistry. Electrophysiological experiments revealed that these beneficial effects were attributed to improved potassium retention and increased rate of Na+ pumping from the cell. The lack of correlation between rutin-induced changes in K+ and H+ fluxes suggest that rutin accumulation in the cytosol scavenges hydroxyl radical formed in response to salinity treatment thus preventing K+ leak via one of ROS-activated K+ efflux pathways, rather than controlling K+ flux via voltage-gated K+-permeable channels.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | salinity, potassium, sodium, mesophyll, sequestration |
| Research Division: | Biological Sciences |
| Research Group: | Plant biology |
| Research Field: | Plant physiology |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding knowledge |
| Objective Field: | Expanding knowledge in the environmental sciences |
| UTAS Author: | Ismail, H (Dr Hebatallah Ismail) |
| UTAS Author: | Shabala, L (Associate Professor Lana Shabala) |
| UTAS Author: | Zivanovic, BD (Dr Branka Zivanovic) |
| UTAS Author: | Tian, Y (Ms Yu Tian) |
| UTAS Author: | Jacobsen, S-E (Associate Professor Sven-Erik Jacobsen) |
| UTAS Author: | Shabala, S (Professor Sergey Shabala) |
| ID Code: | 107577 |
| Year Published: | 2016 |
| Web of Science® Times Cited: | 56 |
| Deposited By: | Tasmanian Institute of Agriculture |
| Deposited On: | 2016-03-18 |
| Last Modified: | 2017-11-02 |
| Downloads: | 0 |
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