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A new allele for aluminium tolerance gene in barley (Hordeum vulgare L.)

Citation

Ma, Y and Li, C and Ryan, PR and Shabala, SN and You, J and Liu, J and Liu, C and Zhou, M, A new allele for aluminium tolerance gene in barley (Hordeum vulgare L.), BMC Genomics, 17, (1) Article 186. ISSN 1471-2164 (2016) [Refereed Article]


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Copyright Statement

2016 Ma et al. Licensed under Creative Commons Attribution 4.0 International (CC BY 4) http://creativecommons.org/licenses/by/4.0/

DOI: doi:10.1186/s12864-016-2551-3

Abstract

Background: Aluminium (Al) toxicity is the main factor limiting the crop production in acid soils and barley (Hordeum vulgare L.) is one of the most Al-sensitive of the small-grained cereals. The major gene for Al tolerance in barley is HvAACT1 (HvMATE) on chromosome 4H which encodes a multidrug and toxic compound extrusion (MATE) protein. The HvAACT1 protein facilitates the Al-activated release of citrate from root apices which protects the growing cells and enables root elongation to continue. A 1 kb transposable element-like insert in the 5 untranslated region (UTR) of HvAACT1 is associated with increased gene expression and tolerance and a PCR-based marker is available to score for this insertion.

Results: We screened a wide range of barley genotypes for Al tolerance and identified a moderately tolerant Chinese genotype named CXHKSL which did not show the typical allele in the 5 UTR of HvAACT1 associated with tolerance. We investigated the mechanism of Al tolerance in CXHKSL and concluded it also relies on the Al-activated release of citrate from roots. Quantitative trait loci (QTL) analysis of double haploid lines generated with CXHKSL and the Al-sensitive variety Gairdner mapped the tolerance locus to the same region as HvAACT1 on chromosome 4H.

Conclusions: Our results show that the Chinese barley genotype CXHKSL possesses a novel allele of the major Al tolerance gene HvAACT1.

Item Details

Item Type:Refereed Article
Keywords:barley, acid soil, QTL, aluminium tolerance, HvAACT1
Research Division:Agricultural and Veterinary Sciences
Research Group:Crop and Pasture Production
Research Field:Crop and Pasture Improvement (Selection and Breeding)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Winter Grains and Oilseeds
Objective Field:Barley
Author:Ma, Y (Ms Yanling Ma)
Author:Shabala, SN (Professor Sergey Shabala)
Author:Liu, J (Mrs Jie Liu)
Author:Zhou, M (Associate Professor Meixue Zhou)
ID Code:107138
Year Published:2016
Web of Science® Times Cited:2
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2016-03-05
Last Modified:2017-11-06
Downloads:82 View Download Statistics

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