Ma, Y and Li, C and Ryan, PR and Shabala, SN and You, J and Liu, J and Liu, C and Zhou, M, A new allele for aluminium tolerance gene in barley (Hordeum vulgare L.), BMC Genomics, 17, (1) Article 186. ISSN 1471-2164 (2016) [Refereed Article]
© 2016 Ma et al. Licensed under Creative Commons Attribution 4.0 International (CC BY 4) http://creativecommons.org/licenses/by/4.0/
Background: Aluminium (Al) toxicity is the main factor limiting the crop production in acid soils and barley (Hordeum vulgare L.) is one of the most Al-sensitive of the small-grained cereals. The major gene for Al tolerance in barley is HvAACT1 (HvMATE) on chromosome 4H which encodes a multidrug and toxic compound extrusion (MATE) protein. The HvAACT1 protein facilitates the Al-activated release of citrate from root apices which protects the growing cells and enables root elongation to continue. A 1 kb transposable element-like insert in the 5’ untranslated region (UTR) of HvAACT1 is associated with increased gene expression and tolerance and a PCR-based marker is available to score for this insertion.
Results: We screened a wide range of barley genotypes for Al tolerance and identified a moderately tolerant Chinese genotype named CXHKSL which did not show the typical allele in the 5’ UTR of HvAACT1 associated with tolerance. We investigated the mechanism of Al tolerance in CXHKSL and concluded it also relies on the Al-activated release of citrate from roots. Quantitative trait loci (QTL) analysis of double haploid lines generated with CXHKSL and the Al-sensitive variety Gairdner mapped the tolerance locus to the same region as HvAACT1 on chromosome 4H.
Conclusions: Our results show that the Chinese barley genotype CXHKSL possesses a novel allele of the major Al tolerance gene HvAACT1.
|Item Type:||Refereed Article|
|Keywords:||barley, acid soil, QTL, aluminium tolerance, HvAACT1|
|Research Division:||Agricultural, Veterinary and Food Sciences|
|Research Group:||Crop and pasture production|
|Research Field:||Crop and pasture improvement (incl. selection and breeding)|
|Objective Division:||Plant Production and Plant Primary Products|
|Objective Group:||Grains and seeds|
|UTAS Author:||Ma, Y (Ms Yanling Ma)|
|UTAS Author:||Shabala, SN (Professor Sergey Shabala)|
|UTAS Author:||Liu, J (Mrs Jie Liu)|
|UTAS Author:||Zhou, M (Professor Meixue Zhou)|
|Web of Science® Times Cited:||18|
|Deposited By:||Tasmanian Institute of Agriculture|
|Downloads:||174 View Download Statistics|
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