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Calibration of the HemoCue point-of-care analyser for determining hemoglobin concentration in a lizard and a fish
Citation
Andrewartha, SJ and Munns, SL and Edwards, A, Calibration of the HemoCue point-of-care analyser for determining hemoglobin concentration in a lizard and a fish, Conservation Physiology, 4, (1) pp. 1-6. ISSN 2051-1434 (2016) [Refereed Article]
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Copyright Statement
Copyright 2016 The Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
DOI: doi:10.1093/conphys/cow006
Abstract
Hemoglobin concentration [Hb] is measured for a wide variety of animal studies. The use of Point-of-care devices, such as the HemoCue, is becoming increasingly common due to their portability, relative ease of use and low cost. This study aimed to determine whether the [Hb] of blue-tongued skink (Tiliqua nigrolutea) blood can be accurately determined using the HemoCue and whether the HemoCue overestimates [Hb] of reptile blood in a similar manner to fish blood. We additionally aimed to test whether ploidy affected [Hb] determined by the HemoCue using blood from diploid and triploid Atlantic salmon (Salmo salar). The HemoCue Hb 201+ systematically overestimated [Hb] in both blue-tongued skinks and Atlantic salmon and there was no difference between calibration equations determined for diploid or triploid salmon. The overestimation was systematic in both species, and as such, [Hb] determined by the HemoCue can be corrected using appropriate calibration equations.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | diploid, reptile, triploid, hemocue validation, haemoglobin, lizard, fish |
| Research Division: | Biological Sciences |
| Research Group: | Other Biological Sciences |
| Research Field: | Biological Sciences not elsewhere classified |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding Knowledge |
| Objective Field: | Expanding Knowledge in the Biological Sciences |
| Author: | Andrewartha, SJ (Dr Sarah Andrewartha) |
| Author: | Edwards, A (Dr Ashley Edwards) |
| ID Code: | 106982 |
| Year Published: | 2016 |
| Web of Science® Times Cited: | 2 |
| Deposited By: | Zoology |
| Deposited On: | 2016-02-29 |
| Last Modified: | 2017-10-31 |
| Downloads: | 67 View Download Statistics |
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