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Calibration of the HemoCue point-of-care analyser for determining hemoglobin concentration in a lizard and a fish


Andrewartha, SJ and Munns, SL and Edwards, A, Calibration of the HemoCue point-of-care analyser for determining hemoglobin concentration in a lizard and a fish, Conservation Physiology, 4, (1) pp. 1-6. ISSN 2051-1434 (2016) [Refereed Article]


Copyright Statement

Copyright 2016 The Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0)

DOI: doi:10.1093/conphys/cow006


Hemoglobin concentration [Hb] is measured for a wide variety of animal studies. The use of Point-of-care devices, such as the HemoCue, is becoming increasingly common due to their portability, relative ease of use and low cost. This study aimed to determine whether the [Hb] of blue-tongued skink (Tiliqua nigrolutea) blood can be accurately determined using the HemoCue and whether the HemoCue overestimates [Hb] of reptile blood in a similar manner to fish blood. We additionally aimed to test whether ploidy affected [Hb] determined by the HemoCue using blood from diploid and triploid Atlantic salmon (Salmo salar). The HemoCue Hb 201+ systematically overestimated [Hb] in both blue-tongued skinks and Atlantic salmon and there was no difference between calibration equations determined for diploid or triploid salmon. The overestimation was systematic in both species, and as such, [Hb] determined by the HemoCue can be corrected using appropriate calibration equations.

Item Details

Item Type:Refereed Article
Keywords:diploid, reptile, triploid, hemocue validation, haemoglobin, lizard, fish
Research Division:Biological Sciences
Research Group:Other biological sciences
Research Field:Other biological sciences not elsewhere classified
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biological sciences
UTAS Author:Andrewartha, SJ (Dr Sarah Andrewartha)
UTAS Author:Edwards, A (Dr Ashley Edwards)
ID Code:106982
Year Published:2016
Web of Science® Times Cited:7
Deposited By:Zoology
Deposited On:2016-02-29
Last Modified:2017-10-31
Downloads:155 View Download Statistics

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