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Stacking in a continuous sample flow interface in capillary electrophoresis


Gstoettenmayr, D and Quirino, J and Ivory, CF and Breadmore, M, Stacking in a continuous sample flow interface in capillary electrophoresis, Journal of Chromatography A, 1408 pp. 236-242. ISSN 0021-9673 (2015) [Refereed Article]

Copyright Statement

Copyright 2015 Elsevier B.V.

DOI: doi:10.1016/j.chroma.2015.06.040


Using a tee connector in a commercial capillary electrophoresis instrument, the effect of field amplified sample injection from both flowing and static sample volumes was investigated. It is shown that under identical conditions (40 min electrokinetic injection at 5 kV from a sample volume of 295 μL) the limit of detection using the continuous sample flow interface is 4 times lower than from a static vial. The relationship between different flow rates and injection voltages on the injected sample amount was also investigated using a 2D axisymmetric simulation (COMSOL 4.3b) and verified experimentally, confirming conditions under which there is near-quantitative injection of the sample target ions. Using electrokinetic injection at 30 kV and a flow rate of 558 nL/s the same enhancement from an even smaller volume of 184 μL could be achieved in 5.5 min than could be achieved from 295 μL and a 40 min injection. This sensitivity enhancement factor corresponded to four orders of magnitude improvement compared to a hydrodynamic injection. This is the first report showing that a continuous sample flow interface combined with stacking methods under conditions approaching quantitative injection from the entire sample volume has the potential to be more sensitive than a static system.

Item Details

Item Type:Refereed Article
Keywords:stacking, flow interface, continuous, sweeping, flowing, interface, electrophoresis
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Gstoettenmayr, D (Mr Daniel Gstoettenmayr)
UTAS Author:Quirino, J (Associate Professor Lito Quirino)
UTAS Author:Breadmore, M (Professor Michael Breadmore)
ID Code:106970
Year Published:2015
Funding Support:Australian Research Council (FT130100101)
Web of Science® Times Cited:8
Deposited By:Chemistry
Deposited On:2016-02-28
Last Modified:2017-10-31

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