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3D printed titanium micro-bore columns containing polymer monoliths for reversed-phase liquid chromatography

journal contribution
posted on 2023-05-18, 17:20 authored by Vipul GuptaVipul Gupta, Mohammad TalebiMohammad Talebi, Deverell, J, Sandron, S, Nesterenko, PN, Heery, B, Thompson, F, Beirne, S, Wallace, GG, Brett PaullBrett Paull
The potential of 3D selective laser melting (SLM) technology to produce compact, temperature and pressure stable titanium alloy chromatographic columns is explored. A micro bore channel (0.9 mm I.D. × 600 mm long) was produced within a 5 × 30 × 30 mm titanium alloy (Ti–6Al–4V) cuboid, in form of a double handed spiral. A poly(butyl methacrylate-co-ethyleneglycoldimethacrylate) (BuMA-co-EDMA) monolithic stationary phase was thermally polymerised within the channel for application in reversed-phase high-performance liquid chromatography. The prepared monolithic column was applied to the liquid chromatographic separation of intact proteins and peptides. Peak capacities of 69–76 (for 6–8 proteins respectively) were observed during isothermal separation of proteins at 44 °C which were further increased to 73–77 using a thermal step gradient with programmed temperature from 60 °C to 35 °C using an in-house built direct-contact heater/cooler platform based upon matching sized Peltier thermoelectric modules. Rapid temperature gradients were possible due to direct-contact between the planar metal column and the Peltier module, and the high thermal conductivity of the titanium column as compared to a similar stainless steel printed column. The separation of peptides released from a digestion of E.coli was also achieved in less than 35 min with ca. 40 distinguishable peaks at 210 nm.

Funding

Department of Industry, Science, Energy and Resources

History

Publication title

Analytica Chimica Acta

Volume

910

Pagination

84-94

ISSN

0003-2670

Department/School

School of Natural Sciences

Publisher

Elsevier BV

Place of publication

Netherlands

Rights statement

Copyright 2016 Elsevier B.V.

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the chemical sciences

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