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3D printed titanium micro-bore columns containing polymer monoliths for reversed-phase liquid chromatography


Gupta, V and Talebi, M and Deverell, J and Sandron, S and Nesterenko, PN and Heery, B and Thompson, F and Beirne, S and Wallace, GG and Paull, B, 3D printed titanium micro-bore columns containing polymer monoliths for reversed-phase liquid chromatography, Analytica Chimica Acta, 910 pp. 84-94. ISSN 0003-2670 (2016) [Refereed Article]

Copyright Statement

Copyright 2016 Elsevier B.V.

DOI: doi:10.1016/j.aca.2016.01.012


The potential of 3D selective laser melting (SLM) technology to produce compact, temperature and pressure stable titanium alloy chromatographic columns is explored. A micro bore channel (0.9mm I.D.נ600mm long) was produced within a 5נ30נ30mm titanium alloy (Ti6Al4V) cuboid, in form of a double handed spiral. A poly(butyl methacrylate-co-ethyleneglycoldimethacrylate) (BuMA-co-EDMA) monolithic stationary phase was thermally polymerised within the channel for application in reversed-phase high-performance liquid chromatography. The prepared monolithic column was applied to the liquid chromatographic separation of intact proteins and peptides. Peak capacities of 6976 (for 68 proteins respectively) were observed during isothermal separation of proteins at 44C which were further increased to 7377 using a thermal step gradient with programmed temperature from 60C to 35C using an in-house built direct-contact heater/cooler platform based upon matching sized Peltier thermoelectric modules. Rapid temperature gradients were possible due to direct-contact between the planar metal column and the Peltier module, and the high thermal conductivity of the titanium column as compared to a similar stainless steel printed column. The separation of peptides released from a digestion of E.coli was also achieved in less than 35min with ca. 40 distinguishable peaks at 210nm.

Item Details

Item Type:Refereed Article
Keywords:3D SLM titanium alloy printing, peltier thermoelectric heating/cooling, module, bidirectional temperature control, step thermal gradient
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Gupta, V (Dr Vipul Gupta)
UTAS Author:Talebi, M (Dr Mohammad Talebi)
UTAS Author:Deverell, J (Dr Jeremy Deverell)
UTAS Author:Sandron, S (Dr Sara Sandron)
UTAS Author:Nesterenko, PN (Professor Pavel Nesterenko)
UTAS Author:Heery, B (Mr Brendan Heery)
UTAS Author:Paull, B (Professor Brett Paull)
ID Code:106838
Year Published:2016
Deposited By:Chemistry
Deposited On:2016-02-23
Last Modified:2017-10-29

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