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Simple, quantitative method for low molecular weight dissolved organic matter extracted from natural waters based upon high performance counter-current chromatography
journal contribution
posted on 2023-05-18, 17:20 authored by Rojas, A, Sandron, S, Richard WilsonRichard Wilson, Noel DaviesNoel Davies, Paul HaddadPaul Haddad, Robert ShellieRobert Shellie, Nesterenko, PN, Brett PaullBrett PaullA simple, high-performance counter-current chromatography method with sequential UV absorbance (254 nm) and evaporative light scattering detection (ELSD) was developed for the quantification of pre-extracted low molecular weight dissolved organic matter (DOM) extracted from natural waters. The method requires solid-phase extraction (SPE) extraction of only small volumes of water samples, here using poly(styrenedivinylbenzene)-based extraction cartridges (Varian PPL). The extracted and concentrated DOM was quantified using reversed-phase high-performance counter-current chromatography (HPCCC), with a water/methanol (5:5) mobile phase and hexane/ethyl acetate (3:7) stationary phase. The critical chromatographic parameters were optimised, applying a revolution speed of 1900 rpm and a flow-rate of 1 mL min−1. Under these conditions, 50 μL of extracted DOM solution could be injected and quantified using calibration against a reference natural dissolved material (Suwannee River), based upon UV absorbance at 254 nm and ELSD detection. Both detection methods provided excellent linearity (R2 > 0.995) for DOM across the concentration ranges of interest, with limits of detection of 4 μg ml−1 and 7 μg ml−1 for ELSD and UV absorbance, respectively. The method was validated for peak area precision (<5%), and accuracy and recovery based upon spiking seawater samples prior to extraction, together with DOM solutions post-extraction (>95% recovery). The developed method was applied to the determination of the concentration of DOM in seawater, based upon initial sample volumes as small as 20 mL.
Funding
Australian Research Council
History
Publication title
Analytica Chimica ActaVolume
909Pagination
129-138ISSN
0003-2670Department/School
School of Natural SciencesPublisher
Elsevier BVPlace of publication
NetherlandsRights statement
Copyright 2016 Elsevier B.V.Repository Status
- Restricted