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Simple, quantitative method for low molecular weight dissolved organic matter extracted from natural waters based upon high performance counter-current chromatography

Citation

Rojas, A and Sandron, S and Wilson, R and Davies, NW and Haddad, PR and Shellie, RA and Nesterenko, PN and Paull, B, Simple, quantitative method for low molecular weight dissolved organic matter extracted from natural waters based upon high performance counter-current chromatography, Analytica Chimica Acta, 909 pp. 129-138. ISSN 0003-2670 (2016) [Refereed Article]

Copyright Statement

Copyright 2016 Elsevier B.V.

DOI: doi:10.1016/j.aca.2016.01.003

Abstract

A simple, high-performance counter-current chromatography method with sequential UV absorbance (254 nm) and evaporative light scattering detection (ELSD) was developed for the quantification of pre-extracted low molecular weight dissolved organic matter (DOM) extracted from natural waters. The method requires solid-phase extraction (SPE) extraction of only small volumes of water samples, here using poly(styrenedivinylbenzene)-based extraction cartridges (Varian PPL). The extracted and concentrated DOM was quantified using reversed-phase high-performance counter-current chromatography (HPCCC), with a water/methanol (5:5) mobile phase and hexane/ethyl acetate (3:7) stationary phase. The critical chromatographic parameters were optimised, applying a revolution speed of 1900 rpm and a flow-rate of 1 mL min−1. Under these conditions, 50 μL of extracted DOM solution could be injected and quantified using calibration against a reference natural dissolved material (Suwannee River), based upon UV absorbance at 254 nm and ELSD detection. Both detection methods provided excellent linearity (R2 > 0.995) for DOM across the concentration ranges of interest, with limits of detection of 4 μg ml−1 and 7 μg ml−1 for ELSD and UV absorbance, respectively. The method was validated for peak area precision (<5%), and accuracy and recovery based upon spiking seawater samples prior to extraction, together with DOM solutions post-extraction (>95% recovery). The developed method was applied to the determination of the concentration of DOM in seawater, based upon initial sample volumes as small as 20 mL.

Item Details

Item Type:Refereed Article
Keywords:high-performance counter-current chromatography, dissolved organic matter, Suwannee river natural organic matter, quantification, evaporative light scattering detection
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Rojas, A (Mr Alfonso Rojas Cardona)
Author:Sandron, S (Dr Sara Sandron)
Author:Wilson, R (Dr Richard Wilson)
Author:Davies, NW (Associate Professor Noel Davies)
Author:Haddad, PR (Professor Paul Haddad)
Author:Shellie, RA (Associate Professor Robert Shellie)
Author:Nesterenko, PN (Professor Pavel Nesterenko)
Author:Paull, B (Professor Brett Paull)
ID Code:106837
Year Published:2016
Funding Support:Australian Research Council (DP130101518)
Web of Science® Times Cited:5
Deposited By:Chemistry
Deposited On:2016-02-23
Last Modified:2017-10-30
Downloads:0

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