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Molecular characterization of several Wx alleles in durum wheat

Citation

Ortega Martinez, R and Guzman, C and Alvarez, JB, Molecular characterization of several Wx alleles in durum wheat, Biologia Plantarum, 59, (2) pp. 220-226. ISSN 0006-3134 (2015) [Refereed Article]

Copyright Statement

Springer International Publishing AG, Part of Springer Science+Business Media

DOI: doi:10.1007/s10535-015-0507-0

Abstract

The Wx gene, which encodes waxy proteins, is the sole gene responsible for amylose synthesis in the wheat seed endosperm. In this study, we characterized, at the molecular level, several Wx alleles in durum wheat (Triticum turgidum L. ssp. durum Desf.) that had previously been catalogued at the protein level. Our data show a misclassification of the alleles in both Wx genes: Wx-A1 alleles from Blanqueta (Wx-A1a) and Astrodur (Wx-A1b) have been reclassified as Wx-A1a and Wx-A1h, respectively. A sequence comparison of the Wx-B1e allele shows that there were up to five different alleles under this denomination, which confirms that the protein analysis by SDS-PAGE separation needs to be combined with the PCR amplification-sequencing analysis in order to obtain a correct classification of the alleles, which will facilitate their use in wheat quality improvement.

Item Details

Item Type:Refereed Article
Keywords:PCR amplification, SDS-PAGE, Triticum turgidum, waxy proteins
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Biology not elsewhere classified
Objective Division:Plant Production and Plant Primary Products
Objective Group:Winter Grains and Oilseeds
Objective Field:Wheat
Author:Ortega Martinez, R (Mr Raul Ortega Martinez)
ID Code:106749
Year Published:2015
Web of Science® Times Cited:1
Deposited By:Plant Science
Deposited On:2016-02-19
Last Modified:2016-04-15
Downloads:0

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