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Blockage of Brassinosteroid Biosynthesis and Sensitivity Causes Dwarfism in Garden Pea

Citation

Nomura, T and Nakayama, M and Reid, JB and Takeuchi, Y and Yokota, T, Blockage of Brassinosteroid Biosynthesis and Sensitivity Causes Dwarfism in Garden Pea, Plant Physiol, 113, (1) pp. 31-37. ISSN 0032-0889 (1997) [Refereed Article]

DOI: doi:10.1104/pp.113.1.31

Abstract

Endogenous brassinosteroids (BRs) in the dwarf mutants lka and lkb of garden pea (Pisum sativum L.) and comparable wild-type plants were quantified by gas chromatography-selected ion monitoring using deuterated internal standards. In young shoots of the lkb mutant, the levels of brassinolide, castasterone, and 6-deoxocastasterone were 23-, 22-, and 9-fold lower, respectively, than those of wild-type plants. Applications of brassinolide, cas-tasterone, typhasterol, 3-dehydroteasterone, and teasterone normalized internode growth of lkb seedlings. These findings indicate that the lkb plants are BR-deficient mutants, probably as a consequence of a block in the BR biosynthetic pathway prior to the production of teasterone. Young shoots of lka plants contained only 50% less brassinolide and 5 times more castasterone than the equivalent wild-type tissues. The lka seedlings were approximately 100 times less responsive to brassinolide than the lkb mutant, and application of castasterone had only a marginal effect on lka internode growth, suggesting that the lka lesion results in impaired sensitivity to BR.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Genetics
Research Field:Gene Expression (incl. Microarray and other genome-wide approaches)
Objective Division:Information and Communication Services
Objective Group:Other Information and Communication Services
Objective Field:Information and Communication Services not elsewhere classified
Author:Reid, JB (Professor Jim Reid)
ID Code:10664
Year Published:1997
Web of Science® Times Cited:144
Deposited By:Plant Science
Deposited On:1997-08-01
Last Modified:2010-11-08
Downloads:0

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