Ten pairs of near-isogenic lines (NILs) were developed for a major quantitative trait locus conferring Fusarium crown rot (FCR) resistance in barley. The locus locates on the long arm of chromosome 4H and the presence of the resistance allele reduced FCR severity by between 32.8 and 63.4 % with an average of 43.9 % across these NILs. Histological and quantitative PCR analyses all confirmed that the rates of Fusarium infection and disease development were much slower in the resistant isolines compared with those in the susceptible isolines. The contrasting phenotypes in FCR severity between the isolines for each of these NIL pairs would facilitate efforts in cloning and functional analyses of genes conferring resistance to this disease.

Item Type:	Refereed Article
Keywords:	Fusarium crown rot, barley, disease resistance, near isogenic lines
Research Division:	Agricultural, Veterinary and Food Sciences
Research Group:	Crop and pasture production
Research Field:	Crop and pasture improvement (incl. selection and breeding)
Objective Division:	Plant Production and Plant Primary Products
Objective Group:	Grains and seeds
Objective Field:	Wheat
UTAS Author:	Habib, A (Mr Ahsan Habib)
UTAS Author:	Shabala, S (Professor Sergey Shabala)
UTAS Author:	Shabala, L (Associate Professor Lana Shabala)
UTAS Author:	Zhou, M (Professor Meixue Zhou)
ID Code:	106565
Year Published:	2016
Web of Science® Times Cited:	17
Deposited By:	Tasmanian Institute of Agriculture
Deposited On:	2016-02-14
Last Modified:	2017-11-06
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