University of Tasmania
Browse

File(s) under permanent embargo

Experimental diffuse brain injury results in regional alteration of gross vascular morphology independent of neuropathology

journal contribution
posted on 2023-05-18, 16:52 authored by Jenna ZiebellJenna Ziebell, Rowe, RK, Harrison, JL, Eakin, KC, Colburn, T, Willyerd, FA, Lifshitz, J
PRIMARY OBJECTIVE: A dynamic relationship exists between diffuse traumatic brain injury and changes to the neurovascular unit. The purpose of this study was to evaluate vascular changes during the first week following diffuse TBI. It was hypothesized that pathology is associated with modification of the vasculature. METHODS: Male Sprague-Dawley rats underwent either midline fluid percussion injury or sham-injury. Brain tissue was collected 1, 2 or 7 days post-injury or sham-injury (n = 3/time point). Tissue was collected and stained by de Olmos amino-cupric silver technique to visualize neuropathology or animals were perfused with AltaBlue casting resin before high-resolution vascular imaging. The average volume, surface area, radius, branching and tortuosity of the vessels were evaluated across three regions of interest. RESULTS: In M2, average vessel volume (p < 0.01) and surface area (p < 0.05) were significantly larger at 1 day relative to 2 days, 7 days and sham. In S1BF and VPM, no significant differences in the average vessel volume or surface area at any of the post-injury time points were observed. No significant changes in average radius, branching or tortuosity were observed. CONCLUSIONS: Preliminary findings suggest gross morphological changes within the vascular network likely represent an acute response to mechanical forces of injury, rather than delayed or chronic pathological processes.

History

Publication title

Brain Injury

Volume

30

Pagination

217-224

ISSN

0269-9052

Department/School

Wicking Dementia Research Education Centre

Publisher

Taylor & Francis Ltd

Place of publication

United Kingdom

Rights statement

Copyright 2016 Taylor & Francis Group, LLC.

Repository Status

  • Restricted

Socio-economic Objectives

Clinical health not elsewhere classified

Usage metrics

    University Of Tasmania

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC