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The release and uptake of metals from potential biofilm inhibition products during spiny lobster (Sagmariasus verreauxi, H. Milne Edwards 1851) culture

Citation

Smith, GG and Poole, AJ and King, PC and Battaglene, S and Fitzgibbon, Q and de Nys, R, The release and uptake of metals from potential biofilm inhibition products during spiny lobster (Sagmariasus verreauxi, H. Milne Edwards 1851) culture, Aquaculture Research, 48, (2) pp. 608-617. ISSN 1355-557X (2016) [Refereed Article]

Copyright Statement

2015 John Wiley & Sons Ltd

DOI: doi:10.1111/are.12907

Abstract

Zinc (Zn) and copper (Cu) are strong inhibitors of bacterial biofilms in aqueous solutions, but are known toxins of crustaceans. A new metal application method; cold-sprayed metal embedment, known to modulate metal release, was tested for its applications in crustacean larval culture systems. Cold-spray technology allows metal particles to bond to plastics, while modulating metal ion release and biocide activity to the substrate boundary. In this study, Eastern spiny lobster (Sagmariasus verreauxi) larvae (phyllosoma) were cultured in the presence of cold-sprayed Zn and Cu metal surfaces. Metal loss was monitored gravimetrically on embedded surfaces, assessment of water ion concentrations and analysis of phyllosoma body content were undertaken. Phyllosoma moulting, deformity and mortality patterns were monitored. Cold-sprayed Zn- and Cu-embedded surfaces were depleted with losses of 0.69% and 31.2% noted respectively. Culture water concentrations of these metals were elevated and accumulation by phyllosoma occurred. Water Zn concentrations of 18.5μgL−1 were associated with chronic eyestalk moult deformities; the first report of Zn causing a non-lethal moult deformity in crustacean larvae. The Cu surface lost a third of its metal mass with a water concentration of 40μgL−1 causing acute toxicity and localization of composite granules in the midgut gland. Cu associated mortality was noted by Day 2 of culture with a LD 50 experienced by Day 9. Future work on the use of bioactive metals in aquaculture systems will focus on a range of different metal alloys, and improved modulation of ion release mechanisms through increased particle embedment depth and separation.

Item Details

Item Type:Refereed Article
Keywords:biofilm, metal inhibition, phyllosoma, larval culture
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Fisheries - Aquaculture not elsewhere classified
Author:Smith, GG (Associate Professor Gregory Smith)
Author:Battaglene, S (Associate Professor Stephen Battaglene)
Author:Fitzgibbon, Q (Dr Quinn Fitzgibbon)
ID Code:106417
Year Published:2016
Funding Support:Australian Research Council (IH120100032)
Deposited By:Sustainable Marine Research Collaboration
Deposited On:2016-02-10
Last Modified:2017-10-12
Downloads:0

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