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Interlaboratory study to evaluate the robustness of capillary electrophoresis-mass spectrometry for peptide mapping

Citation

Wenz, C and Barbas, C and Lopez-Gonzalvez, A and Garcia, A and Benavente, F and Sanz-Nebot, V and Blanc, T and Freckleton, G and Britz-McKibbin, P and Shanmuganathan, M and de l'Escaille, F and Far, J and Haselberg, R and Huang, S and Huhn, C and Pattky, M and Michels, D and Mou, S and Yang, F and Neusuess, C and Tromsdorf, N and Baidoo, EEK and Keasling, JD and Park, SS, Interlaboratory study to evaluate the robustness of capillary electrophoresis-mass spectrometry for peptide mapping, Journal of Separation Science, 38, (18) pp. 3262-3270. ISSN 1615-9306 (2015) [Refereed Article]

Copyright Statement

Copyright 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

DOI: doi:10.1002/jssc.201500551

Abstract

A collaborative study on the robustness and portability of a capillary electrophoresis-mass spectrometry method for peptide mapping was performed by an international team, consisting of 13 independent laboratories from academia and industry. All participants used the same batch of samples, reagents and coated capillaries to run their assays, whereas they utilized the capillary electrophoresis-mass spectrometry equipment available in their laboratories. The equipment used varied in model, type and instrument manufacturer. Furthermore, different types of sheath-flow capillary electrophoresis-mass spectrometry interfaces were used. Migration time, peak height and peak area of ten representative target peptides of trypsin-digested bovine serum albumin were determined by every laboratory on two consecutive days. The data were critically evaluated to identify outliers and final values for means, repeatability (precision within a laboratory) and reproducibility (precision between laboratories) were established. For relative migration time the repeatability was between 0.05 and 0.18% RSD and the reproducibility between 0.14 and 1.3% RSD. For relative peak area repeatability and reproducibility values obtained were 3-12 and 9-29% RSD, respectively. These results demonstrate that capillary electrophoresis-mass spectrometry is robust enough to allow a method transfer across multiple laboratories and should promote a more widespread use of peptide mapping and other capillary electrophoresis-mass spectrometry applications in biopharmaceutical analysis and related fields.

Item Details

Item Type:Refereed Article
Keywords:precision, repeatability, reproducibility, system suitability test, tryptic digest
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Haselberg, R (Dr Rob Haselberg)
ID Code:106293
Year Published:2015
Web of Science® Times Cited:9
Deposited By:Chemistry
Deposited On:2016-02-04
Last Modified:2016-09-20
Downloads:0

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