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A polymerase chain reaction assay for ascosporic inoculum of Sclerotinia species

journal contribution
posted on 2023-05-18, 16:37 authored by Suzanne JonesSuzanne Jones, Stacey PilkingtonStacey Pilkington, Gent, DH, Hay, FS, Pethybridge, SJ
A polymerase chain reaction (PCR) assay was developed that amplified a 170-bp fragment of the intergenic spacer region of Sclerotinia sclerotiorum, the cause of white mould. Sensitivity was 10 S. sclerotiorum ascospores per DNA extraction (0.2 ascospores per PCR reaction). The presence of soil did not affect sensitivity at 50, 100 and 500 ascospores/DNA extraction, but reduced sensitivity at 25 and 10 ascospores/DNA extraction by 10% and 30%, respectively. The assay did not amplify DNA of Botrytis cinerea but detected S. minor and S. trifoliorum. Utility of the test for detection of S. sclerotiorum ascospores in bean fields was demonstrated using rotating impaction samplers over two seasons. The use of the test in combination with an impaction sampler may provide benefits in time, sensitivity and specificity compared with visual identification and enumeration of spores from traps only. This system may provide an opportunity to schedule fungicides during periods of inoculum presence for disease management.

History

Publication title

New Zealand Journal of Crop and Horticultural Science

Volume

43

Pagination

233-240

ISSN

0114-0671

Department/School

Tasmanian Institute of Agriculture (TIA)

Publisher

Sir Publishing

Place of publication

Po Box 399, Wellington, New Zealand

Rights statement

Copyright 2015 The Royal Society of New Zealand

Repository Status

  • Restricted

Socio-economic Objectives

Field grown vegetable crops

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