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A polymerase chain reaction assay for ascosporic inoculum of Sclerotinia species


Jones, SJ and Pilkington, SJ and Gent, DH and Hay, FS and Pethybridge, SJ, A polymerase chain reaction assay for ascosporic inoculum of Sclerotinia species, New Zealand Journal of Crop and Horticultural Science, 43, (3) pp. 233-240. ISSN 0114-0671 (2015) [Refereed Article]

Copyright Statement

Copyright 2015 The Royal Society of New Zealand

DOI: doi:10.1080/01140671.2015.1025083


A polymerase chain reaction (PCR) assay was developed that amplified a 170-bp fragment of the intergenic spacer region of Sclerotinia sclerotiorum, the cause of white mould. Sensitivity was 10 S. sclerotiorum ascospores per DNA extraction (0.2 ascospores per PCR reaction). The presence of soil did not affect sensitivity at 50, 100 and 500 ascospores/DNA extraction, but reduced sensitivity at 25 and 10 ascospores/DNA extraction by 10% and 30%, respectively. The assay did not amplify DNA of Botrytis cinerea but detected S. minor and S. trifoliorum. Utility of the test for detection of S. sclerotiorum ascospores in bean fields was demonstrated using rotating impaction samplers over two seasons. The use of the test in combination with an impaction sampler may provide benefits in time, sensitivity and specificity compared with visual identification and enumeration of spores from traps only. This system may provide an opportunity to schedule fungicides during periods of inoculum presence for disease management.

Item Details

Item Type:Refereed Article
Keywords:ascospores, bean, disease forecasting, Sclerotinia sclerotiorum, white mould
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Crop and pasture production
Research Field:Crop and pasture protection (incl. pests, diseases and weeds)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Horticultural crops
Objective Field:Field grown vegetable crops
UTAS Author:Jones, SJ (Dr Suzie Jones)
UTAS Author:Pilkington, SJ (Mrs Stacey Pilkington)
UTAS Author:Hay, FS (Dr Frank Hay)
ID Code:106285
Year Published:2015
Web of Science® Times Cited:3
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2016-02-04
Last Modified:2017-11-06

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