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Transient isotachophoresis-capillary zone electrophoresis for the analysis of paralytic shellfish toxins in mussel sample


Abdul Keyon, AS and Guijt, RM and Bolch, CJS and Breadmore, MC, Transient isotachophoresis-capillary zone electrophoresis for the analysis of paralytic shellfish toxins in mussel sample, The 16th International Conference on Harmful Algae Book of Abstracts, 27-31 October, Wellington, New Zealand, pp. 118. (2014) [Conference Extract]

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Accumulation of paralytic shellfish toxins (PSTs) in contaminated shellfish is a serious health risk making early detection important to improve shellfish safety and biotoxin management. Capillary electrophoresis (CE) has been proven as a high resolution separation technique compatible with miniaturization, making it an attractive choice in the development of portable instrumentation for early, on-site detection of PSTs. In this work, capillary zone electrophoresis (CZE) with capacitively coupled contactless conductivity detector (C4D) was compared with UV detection because of its small footprint. To improve sensitivity and deal with the high conductivity sample matrix, counter-flow transient isotachophoresis (tiTP) was used, exploiting the high sodium levels in the matrix as leading ion while L-alanine was used as the background electrolyte (BGE) and terminating electrolyte (TE). Careful optimization of the injected sample volume and duration of the counter-flow resulted in limit of detections (LODs) ranging from 74.2- 1020 ng/ml for tiTP-CZE-C4D and 141-461 ng/ml for tiTP-CZE-UV, an 8-97 fold over reduction compared to conventional CZE. The LODs were adequate for the analysis of PSTs in shellfish samples close to the regulatory limit (800 ng/ml). The developed method was applied to the analysis of a contaminated mussel sample and validated against results from Association of Official Analytical Chemists (AOAC)-approved method for PSTs analysis using pre-column oxidation of the sample prior to analysis by high performance liquid chromatography (HPLC) with fluorescence detection (FLO). The method presented has potential for incorporation into field-deployable devices for the early detection of PSTs on-site.

Item Details

Item Type:Conference Extract
Keywords:capillary electrophoresis, paralytic shellfish, toxins
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Instrumental methods (excl. immunological and bioassay methods)
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - aquaculture
Objective Field:Fisheries - aquaculture not elsewhere classified
UTAS Author:Abdul Keyon, AS (Mrs Aemi Abdul Keyon)
UTAS Author:Guijt, RM (Dr Rosanne Guijt)
UTAS Author:Bolch, CJS (Associate Professor Christopher Bolch)
UTAS Author:Breadmore, MC (Professor Michael Breadmore)
ID Code:106255
Year Published:2014
Deposited By:IMAS Research and Education Centre
Deposited On:2016-02-03
Last Modified:2016-02-03

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