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Quantitative detection of G. catenatum by qPCR based on the rDNA-ITS and sxtA4 genes

Citation

Untari, L and Burke, C and Kunde, D and Murray, S and Bolch, C, Quantitative detection of G. catenatum by qPCR based on the rDNA-ITS and sxtA4 genes, The 16th International Conference on Harmful Algae Book of Abstracts, 27-31 October, Wellington, New Zealand, pp. 59. (2014) [Conference Extract]


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Abstract

Blooms of Gymnodinium catenatum occur annually in southern Tasmania, particularly the Huon Estuary, D'Entrecasteaux Channel and Port Esperance. To detect the presence of G. catenatum in the environment a G. catenatum-specific quantitative PCR assays were established targeting the rDNA-ITS (ITS1-5.8S-ITS2) region and combined with a qPCR targeting the A4 domain of the saxitoxin synthetase gene (sxtA4). The G. catenatum rDNA-ITS primer specificity was tested against related gymnodinoids including G. nolleri and G. microreticulatum and were capable of detecting the presence of the saxitoxin-producing species in the water environment at concentrations below routine light microscopic detection. This assays are being developed as an early warning of the potential PST toxicity in the water environmental.

Item Details

Item Type:Conference Extract
Keywords:dinoflagellate, Gymnodinium, saxitoxin, PST gene, SxtA, DNA
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Phycology (incl. Marine Grasses)
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Fisheries - Aquaculture not elsewhere classified
Author:Untari, L (Mrs Ludmilla Untari)
Author:Burke, C (Dr Chris Burke)
Author:Kunde, D (Dr Dale Kunde)
Author:Bolch, C (Dr Christopher Bolch)
ID Code:106253
Year Published:2014
Deposited By:IMAS Research and Education Centre
Deposited On:2016-02-03
Last Modified:2016-02-03
Downloads:0

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