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Polyketide synthesis genes associated with toxin production in two species of Gambierdiscus (Dinophyceae)

Citation

Kohli, GS and John, U and Figueroa, RI and Rhodes, LL and Harwood, DT and Groth, M and Bolch, CJS and Murray, SA, Polyketide synthesis genes associated with toxin production in two species of Gambierdiscus (Dinophyceae), BMC Genomics, 16, (1) Article 410. ISSN 1471-2164 (2015) [Refereed Article]


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Copyright Statement

© 2015 Kohli et al. Licensed under Creative Commons Attribution 4.0 International (CC BY 4) http://creativecommons.org/licenses/by/4.0/

DOI: doi:10.1186/s12864-015-1625-y

Abstract

Background

Marine microbial protists, in particular, dinoflagellates, produce polyketide toxins with ecosystem-wide and human health impacts. Species of Gambierdiscus produce the polyether ladder compounds ciguatoxins and maitotoxins, which can lead to ciguatera fish poisoning, a serious human illness associated with reef fish consumption. Genes associated with the biosynthesis of polyether ladder compounds are yet to be elucidated, however, stable isotope feeding studies of such compounds consistently support their polyketide origin indicating that polyketide synthases are involved in their biosynthesis.

Results

Here, we report the toxicity, genome size, gene content and transcriptome of Gambierdiscus australes and G. belizeanus. G. australes produced maitotoxin-1 and maitotoxin-3, while G. belizeanus produced maitotoxin-3, for which cell extracts were toxic to mice by IP injection (LD50 = 3.8 mg kg-1). The gene catalogues comprised 83,353 and 84,870 unique contigs, with genome sizes of 32.5 ± 3.7 Gbp and 35 ± 0.88 Gbp, respectively, and are amongst the most comprehensive yet reported from a dinoflagellate. We found three hundred and six genes involved in polyketide biosynthesis, including one hundred and ninty-two ketoacyl synthase transcripts, which formed five unique phylogenetic clusters.

Conclusions

Two clusters were unique to these maitotoxin-producing dinoflagellate species, suggesting that they may be associated with maitotoxin biosynthesis. This work represents a significant step forward in our understanding of the genetic basis of polyketide production in dinoflagellates, in particular, species responsible for ciguatera fish poisoning.

Item Details

Item Type:Refereed Article
Keywords:Gambierdiscus, ciguatera, ciguatoxin, maitotoxin, polyketide, synthesis gene
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Phycology (incl. Marine Grasses)
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Wild Caught
Objective Field:Fisheries - Wild Caught not elsewhere classified
Author:Bolch, CJS (Dr Christopher Bolch)
ID Code:106248
Year Published:2015
Funding Support:Australian Research Council (DP0880264)
Web of Science® Times Cited:7
Deposited By:IMAS Research and Education Centre
Deposited On:2016-02-03
Last Modified:2016-05-19
Downloads:33 View Download Statistics

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