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Genetic diversity of culturable Vibrio in an Australian blue mussel Mytilus galloprovincialis hatchery

Citation

Kwan, TN and Bolch, CJS, Genetic diversity of culturable Vibrio in an Australian blue mussel Mytilus galloprovincialis hatchery, Diseases of Aquatic Organisms, 116, (1) pp. 37-46. ISSN 0177-5103 (2015) [Refereed Article]

Copyright Statement

Inter-Research 2015 www.int-res.com

DOI: doi:10.3354/dao02905

Abstract

Bacillary necrosis associated with Vibrio species is the common cause of larval and spat mortality during commercial production of Australian blue mussel Mytilus galloprovincialis. A total of 87 randomly selected Vibrio isolates from various stages of rearing in a commercial mussel hatchery were characterised using partial sequences of the ATP synthase alpha subunit gene (atpA). The sequenced isolates represented 40 unique atpA genotypes, overwhelmingly dominated (98%) by V. splendidus group genotypes, with 1 V. harveyi group genotype also detected. The V. splendidus group sequences formed 5 moderately supported clusters allied with V. splendidus/V. lentus, V. atlanticus, V. tasmaniensis, V. cyclitrophicus and V. toranzoniae. All water sources showed considerable atpA gene diversity among Vibrio isolates, with 30 to 60% of unique isolates recovered from each source. Over half (53%) of Vibrio atpA genotypes were detected only once, and only 7 genotypes were recovered from multiple sources. Comparisons of phylogenetic diversity using UniFrac analysis showed that the culturable Vibrio community from intake, header, broodstock and larval tanks were phylogenetically similar, while spat tank communities were different. Culturable Vibrio associated with spat tank seawater differed in being dominated by V. toranzoniae-affiliated genotypes. The high diversity of V. splendidus group genotypes detected in this study reinforces the dynamic nature of microbial communities associated with hatchery culture and complicates our efforts to elucidate the role of V. splendidus group bacteria in vibriosis.

Item Details

Item Type:Refereed Article
Keywords:blue mussels, Vibrio, hatchery, diversity, DNA
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Fish Pests and Diseases
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Aquaculture Molluscs (excl. Oysters)
Author:Kwan, TN (Mr Tzu Kwan)
Author:Bolch, CJS (Dr Christopher Bolch)
ID Code:106247
Year Published:2015
Web of Science® Times Cited:5
Deposited By:IMAS Research and Education Centre
Deposited On:2016-02-03
Last Modified:2017-11-04
Downloads:0

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