eCite Digital Repository
Antarctic krill population genomics: apparent panmixia, but genome complexity and large population size muddy the water
Citation
Deagle, BE and Faux, C and Kawaguchi, S and Meyer, B and Jarman, SN, Antarctic krill population genomics: apparent panmixia, but genome complexity and large population size muddy the water, Molecular Ecology, 24, (19) pp. 4943-4959. ISSN 0962-1083 (2015) [Refereed Article]
Copyright Statement
© 2015 John Wiley
Abstract
Antarctic krill (Euphausia superba; hereafter krill) are an incredibly abundant pelagic
crustacean which has a wide, but patchy, distribution in the Southern Ocean. Several
studies have examined the potential for population genetic structuring in krill, but
DNA-based analyses have focused on a limited number of markers and have covered
only part of their circum-Antarctic range. We used mitochondrial DNA and restriction
site-associated DNA sequencing (RAD-seq) to investigate genetic differences between
krill from five sites, including two from East Antarctica. Our mtDNA results show no
discernible genetic structuring between sites separated by thousands of kilometres,
which is consistent with previous studies. Using standard RAD-seq methodology, we
obtained over a billion sequences from >140 krill, and thousands of variable nucleotides
were identified at hundreds of loci. However, downstream analysis found that
markers with sufficient coverage were primarily from multicopy genomic regions.
Careful examination of these data highlights the complexity of the RAD-seq approach
in organisms with very large genomes. To characterize the multicopy markers, we
recorded sequence counts from variable nucleotide sites rather than the derived genotypes;
we also examined a small number of manually curated genotypes. Although
these analyses effectively fingerprinted individuals, and uncovered a minor laboratory
batch effect, no population structuring was observed. Overall, our results are consistent
with panmixia of krill throughout their distribution. This result may indicate
ongoing gene flow. However, krill’s enormous population size creates substantial panmictic
inertia, so genetic differentiation may not occur on an ecologically relevant timescale
even if demographically separate populations exist.
Item Details
Item Type: | Refereed Article |
---|---|
Keywords: | genotyping by sequencing, RAD sequencing, repetitive DNA, zooplankton |
Research Division: | Biological Sciences |
Research Group: | Genetics |
Research Field: | Genetics not elsewhere classified |
Objective Division: | Environmental Management |
Objective Group: | Coastal and estuarine systems and management |
Objective Field: | Assessment and management of coastal and estuarine ecosystems |
UTAS Author: | Deagle, BE (Dr Bruce Deagle) |
UTAS Author: | Kawaguchi, S (Dr So Kawaguchi) |
ID Code: | 106031 |
Year Published: | 2015 |
Web of Science® Times Cited: | 24 |
Deposited By: | CRC-Antarctic Climate & Ecosystems |
Deposited On: | 2016-01-25 |
Last Modified: | 2017-11-01 |
Downloads: | 0 |
Repository Staff Only: item control page