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Discovery of conventional prolactin from the holocephalan elephant fish, Callorhinchus milii

Citation

Yamaguchi, Y and Takagi, W and Kuraku, S and Moriyama, S and Bell, JD and Seale, AP and Lerner, DT and Grau, EG and Hyodo, S, Discovery of conventional prolactin from the holocephalan elephant fish, Callorhinchus milii, General and Comparative Endocrinology, 224 pp. 216-227. ISSN 0016-6480 (2015) [Refereed Article]

Copyright Statement

Copyright 2015 Elsevier Inc.

DOI: doi:10.1016/j.ygcen.2015.08.020

Abstract

The conventional prolactin (PRL), also known as PRL1, is an adenohypophysial hormone that critically regulates various physiological events in reproduction, metabolism, growth, osmoregulation, among others. PRL1 shares its evolutionary origin with PRL2, growth hormone (GH), somatolactin and placental lactogen, which together form the GH/PRL hormone family. Previously, several bioassays implied the existence of PRL1 in elasmobranch pituitaries. However, to date, all attempts to isolate PRL1 from chondrichthyans have been unsuccessful. Here, we cloned PRL1 from the pituitary of the holocephalan elephant fish, Callorhinchus milii, as the first report of chondrichthyan PRL1. The putative mature protein of elephant fish PRL1 (cmPRL1) consists of 198 amino acids, containing two conserved disulfide bonds. The orthologous relationship of cmPRL1 to known vertebrate PRL1s was confirmed by the analyses of molecular phylogeny and gene synteny. The cmPRL1 gene was similar to teleost PRL1 genes in gene synteny, but was distinct from amniote PRL1 genes, which most likely arose in an early amphibian by duplication of the ancestral PRL1 gene. The mRNA of cmPRL1 was predominantly expressed in the pituitary, but was considerably less abundant than has been previously reported for bony fish and tetrapod PRL1s; the copy number of cmPRL1 mRNA in the pituitary was less than 1% and 0.1% of that of GH and pro-opiomelanocortin mRNAs, respectively. The cells expressing cmPRL1 mRNA were sparsely distributed in the rostral pars distalis. Our findings provide a new insight into the studies on molecular and functional evolution of PRL1 in vertebrates.

Item Details

Item Type:Refereed Article
Keywords:prolactin, GH/PRL family, cartilaginous fish, molecular evolution
Research Division:Biological Sciences
Research Group:Genetics
Research Field:Molecular Evolution
Objective Division:Environment
Objective Group:Flora, Fauna and Biodiversity
Objective Field:Marine Flora, Fauna and Biodiversity
Author:Bell, JD (Dr Justin Bell)
ID Code:106023
Year Published:2015
Web of Science® Times Cited:1
Deposited By:IMAS Research and Education Centre
Deposited On:2016-01-25
Last Modified:2017-10-31
Downloads:0

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