File(s) under permanent embargo
Estimation of the uncertainty of analyte concentration from the measurement uncertainty
journal contribution
posted on 2023-05-18, 16:15 authored by Brown, S, Cooke, DG, Blackwell, LFLigand-binding assays, such as immunoassays, are usually analysed using standard curves based on the four-parameter and five-parameter logistic models. An estimate of the uncertainty of an analyte concentration obtained from such curves is needed for confidence intervals or precision profiles. Using a numerical simulation approach, it is shown that the uncertainty of the analyte concentration estimate becomes significant at the extremes of the concentration range and that this is affected significantly by the steepness of the standard curve. We also provide expressions for the coefficient of variation of the analyte concentration estimate from which confidence intervals and the precision profile can be obtained. Using three examples, we show that the expressions perform well.
History
Publication title
Scandinavian Journal of Clinical and Laboratory InvestigationVolume
75Issue
5Pagination
367-373ISSN
0036-5513Department/School
School of Health SciencesPublisher
Taylor & Francis AsPlace of publication
NorwayRights statement
© 2015 Informa HealthcareRepository Status
- Restricted