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Estimation of the uncertainty of analyte concentration from the measurement uncertainty


Brown, S and Cooke, DG and Blackwell, LF, Estimation of the uncertainty of analyte concentration from the measurement uncertainty, Scandinavian Journal of Clinical and Laboratory Investigation, 75, (5) pp. 367-373. ISSN 0036-5513 (2015) [Refereed Article]

Copyright Statement

2015 Informa Healthcare

DOI: doi:10.3109/00365513.2015.1031688


Ligand-binding assays, such as immunoassays, are usually analysed using standard curves based on the four-parameter and five-parameter logistic models. An estimate of the uncertainty of an analyte concentration obtained from such curves is needed for confidence intervals or precision profiles. Using a numerical simulation approach, it is shown that the uncertainty of the analyte concentration estimate becomes significant at the extremes of the concentration range and that this is affected significantly by the steepness of the standard curve. We also provide expressions for the coefficient of variation of the analyte concentration estimate from which confidence intervals and the precision profile can be obtained. Using three examples, we show that the expressions perform well.

Item Details

Item Type:Refereed Article
Keywords:analytical chemistry methods, clinical chemistry tests, immunoassay, logistic models, uncertainty
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Analytical chemistry not elsewhere classified
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Brown, S (Dr Simon Brown)
ID Code:105989
Year Published:2015
Web of Science® Times Cited:1
Deposited By:Health Sciences
Deposited On:2016-01-22
Last Modified:2016-06-02

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