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Regular postexercise cooling enhances mitochondrial biogenesis through AMPK and p38 MAPK in human skeletal muscle
Citation
Ihsan, M and Markworth, JF and Watson, G and Choo, HC and Govus, A and Pham, T and Hickey, A and Cameron-Smith, D and Abbiss, CR, Regular postexercise cooling enhances mitochondrial biogenesis through AMPK and p38 MAPK in human skeletal muscle, AJP: Regulatory, Integrative and Comparative Physiology, 309, (3) pp. R286-R294. ISSN 1522-1490 (2015) [Refereed Article]
Copyright Statement
© 2015 the American Physiological Society
DOI: doi:10.1152/ajpregu.00031.2015
Abstract
This study investigated the effect of regular postexercise cold water immersion (CWI) on muscle aerobic adaptations to endurance training. Eight males performed 3 sessions/wk of endurance training for 4 wk. Following each session, subjects immersed one leg in a cold water bath (10°C; COLD) for 15 min, while the contralateral leg served as a control (CON). Muscle biopsies were obtained from vastus lateralis of both CON and COLD legs prior to training and 48 h following the last training session. Samples were analyzed for signaling kinases: p38 MAPK and AMPK, peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α), enzyme activities indicative of mitochondrial biogenesis, and protein subunits representative of respiratory chain complexes I–V. Following training, subjects' peak oxygen uptake and running velocity were improved by 5.9% and 6.2%, respectively (P < 0.05). Repeated CWI resulted in higher total AMPK, phosphorylated AMPK, phosphorylated acetyl-CoA carboxylase, β-3-hydroxyacyl-CoA-dehydrogenase and the protein subunits representative of complex I and III (P < 0.05). Moreover, large effect sizes (Cohen's d > 0.8) were noted with changes in protein content of p38 (d = 1.02, P = 0.064), PGC-1α (d = 0.99, P = 0.079), and peroxisome proliferator-activated receptor α (d = 0.93, P = 0.10) in COLD compared with CON. No differences between conditions were observed in the representative protein subunits of respiratory complexes II, IV, and V and in the activities of several mitochondrial enzymes (P > 0.05). These findings indicate that regular CWI enhances p38, AMPK, and possibly mitochondrial biogenesis.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | cold water immersion, exercise recovery, muscle oxidative adaptations, nonshivering thermogenesis, PGC-1α |
| Research Division: | Medical and Health Sciences |
| Research Group: | Human Movement and Sports Science |
| Research Field: | Exercise Physiology |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding Knowledge |
| Objective Field: | Expanding Knowledge in the Medical and Health Sciences |
| Author: | Watson, G (Dr Greig Watson) |
| ID Code: | 105970 |
| Year Published: | 2015 |
| Web of Science® Times Cited: | 13 |
| Deposited By: | Health Sciences |
| Deposited On: | 2016-01-21 |
| Last Modified: | 2017-10-31 |
| Downloads: | 0 |
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