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Interface-free two-dimensional heart-cutting capillary electrophoresis for the separation and stacking of anionic and neutral analytes

Citation

Kukusamude, C and Srijaranai, S and Quirino, JP, Interface-free two-dimensional heart-cutting capillary electrophoresis for the separation and stacking of anionic and neutral analytes, Journal of Separation Science, 38, (14) pp. 2532-2537. ISSN 1615-9306 (2015) [Refereed Article]

Copyright Statement

Copyright 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

DOI: doi:10.1002/jssc.201500268

Abstract

Interface-free two-dimensional heart-cutting capillary electrophoresis for two different classes of analytes (anionic and neutral) in a single capillary is presented. Simultaneous sample stacking and orthogonal separation were demonstrated. The anionic species were first analyzed by capillary zone electrophoresis in the first dimension. Then, the neutral compounds were separated in the second dimension by micellar electrokinetic chromatography using the common anionic surfactant sodium dodecyl sulfate. The first and second dimensions occurred automatically without changing the electrolyte and without polarity switching. Artificial mixtures (five anions and four neutral compounds) were successfully analyzed with sensitivity enhancement factors from 7 to 28. The orthogonal separation was complete within 8 min. Some analytical features and application to a spiked real river water sample were also studied.

Item Details

Item Type:Refereed Article
Keywords:capillary electrophoresis, heart-cutting, multidimensional capillary electrophoresis, on-line sample concentration
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Kukusamude, C (Mr Chunyapuk Kukusamude)
Author:Quirino, JP (Associate Professor Lito Quirino)
ID Code:105937
Year Published:2015
Web of Science® Times Cited:5
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2016-01-20
Last Modified:2017-11-01
Downloads:0

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