Sample preparation by electrophoretic concentration, followed by analysis using sweeping-micellar electrokinetic chromatography, was studied as a green and simple analytical strategy for the trace analysis of cationic drugs in water samples. Electrophoretic concentration was conducted using 50 mmol/L ammonium acetate at pH 5 as acceptor electrolyte. Electrophoretic concentration was performed at 1.0 kV for 50 min and 0.5 kV and 15 min for purified and 10-fold diluted waste water samples, respectively. Sweeping-micellar electrokinetic chromatography was with 100 mmol/L sodium phosphate at pH 2, 100 mmol/L sodium dodecyl sulfate and 27.5%-v/v acetonitrile as separation electrolyte. The separation voltage was −20 kV, UV-detection was at 200 nm, and the acidified concentrate was injected for 36 s at 1 bar (or 72% of the total capillary length, 60 cm). Both purified water and 10-fold diluted waste water exhibited a linear range of two orders of concentration magnitude. The coefficient of determination, and intra- and interday repeatability were 0.991–0.997, 2.5–6.2, and 4.4–9.7%RSD (n = 6), respectively, for purified water. The values were 0.991–0.997, 3.4–7.1, and 8.7–9.8%RSD (n = 6), correspondingly, for 10-fold diluted waste water. The method detection limit was in the range from 0.04–0.09 to 1.20–6.97 ng/mL for purified and undiluted waste water, respectively.

Item Type:	Refereed Article
Keywords:	green sample preparation, electrophoretic concentration, sweeping, MEKC, cationic drugs, aqueous samples
Research Division:	Chemical Sciences
Research Group:	Analytical chemistry
Research Field:	Separation science
Objective Division:	Expanding Knowledge
Objective Group:	Expanding knowledge
Objective Field:	Expanding knowledge in the chemical sciences
UTAS Author:	Wuethrich, A (Mr Alain Wuethrich)
UTAS Author:	Haddad, PR (Professor Paul Haddad)
UTAS Author:	Quirino, JP (Associate Professor Lito Quirino)
ID Code:	105934
Year Published:	2015
Web of Science® Times Cited:	10
Deposited By:	Austn Centre for Research in Separation Science
Deposited On:	2016-01-20
Last Modified:	2017-10-30
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