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Determination of tamoxifen and its metabolites in human plasma by nonaqueous capillary electrophoresis with contactless conductivity detection

Citation

Thang, LY and Shahir, S and See, HH, Determination of tamoxifen and its metabolites in human plasma by nonaqueous capillary electrophoresis with contactless conductivity detection, Electrophoresis, 36, (21-22) pp. 2713-2719. ISSN 0173-0835 (2015) [Refereed Article]

Copyright Statement

Copyright 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

DOI: doi:10.1002/elps.201500164

Abstract

A new approach for the quantification of tamoxifen and its metabolites 4-hydroxytamoxifen, N-desmethyltamoxifen, and 4-hydroxy-N-desmethyltamoxifen (endoxifen) in human plasma samples using NACE coupled with contactless conductivity detection (C4D) is presented. The buffer system employed consisted of 7.5 mM deoxycholic acid sodium salt, 15 mM acetic acid, and 1 mM 18-crown-6 in 100% methanol. The complete separation of all targeted compounds (including endoxifen racemate) could be achieved within 6 min under optimized conditions. The proposed method was validated and showed good linearity in the range from 100 to 5000 ng/mL with correlation coefficients between 0.9922 and 0.9973, LODs in the range of 2540 ng/mL, and acceptable reproducibility of the peak area (intraday RSD 2.23.1%, n = 4; interday (3 days) RSD 6.08.8%, n = 4). The developed method was successfully demonstrated for the quantification of tamoxifen and its metabolites in human plasma samples collected from breast cancer patients undertaking tamoxifen treatment.

Item Details

Item Type:Refereed Article
Keywords:breast cancer, contactless conductivity detection, nonaqueous capillary electrophoresis, plasma, tamoxifen
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:See, HH (Dr Hong Heng See)
ID Code:105815
Year Published:2015
Web of Science® Times Cited:7
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2016-01-14
Last Modified:2016-09-21
Downloads:0

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