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Characterization of macromolecular complexes in red wine: composition, molecular mass distribution and particle size


Bindon, KA and Carew, AL and Mierczynska-Vasilev, A and Kassara, S and Kerslake, F and Smith, PA, Characterization of macromolecular complexes in red wine: composition, molecular mass distribution and particle size, Food Chemistry, 199 pp. 838-846. ISSN 0308-8146 (2016) [Refereed Article]

Copyright Statement

Copyright 2015 Published by Elsevier Ltd.

DOI: doi:10.1016/j.foodchem.2015.12.079


Precipitates were prepared from two compositionally different Pinot noir wines with addition of excess ethanol, and contained primarily polysaccharide, tannin and protein. The ethanol-soluble material was further fractionated into polymeric (tannin) and monomeric phenolics. Tannin associated with precipitates was of a higher molecular mass than that remaining in ethanolic solution. Wine fractions were reconstituted at the ratios of the original wine and analyzed using nanoparticle tracking analysis. The average particle size of the tannin fraction was 75–89 nm, and increased when combined with the precipitate (≅200 nm). Addition of the monomeric fraction to the tannin–precipitate complex increased both the incidence and concentration of smaller particles, reducing the average particle size. The formation of aggregates occurred in all fractions and only minor differences in particle size distribution were found between wines. Differences in particle concentration between wines appear to be due to differences in the total concentration of macromolecules rather than compositional differences.

Item Details

Item Type:Refereed Article
Keywords:Pinot noir, proanthocyanidin, maceration, polysaccharide, protein, size exclusion chromatography, molecular mass, particle size, nanaoparticle tracking analysis
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Horticultural production
Research Field:Oenology and viticulture
Objective Division:Plant Production and Plant Primary Products
Objective Group:Industrial crops
Objective Field:Wine grapes
UTAS Author:Carew, AL (Dr Anna Carew)
UTAS Author:Kerslake, F (Dr Fiona Kerslake)
ID Code:105803
Year Published:2016
Web of Science® Times Cited:28
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2016-01-14
Last Modified:2017-11-02

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