Tea plant (Camellia sinensis (L.) O. kuntze) is known to be a fluoride (F) and aluminum (Al³⁺) hyper-accumulator. Previous study showed that pre-treatment of Al³⁺ caused a significant increase of F accumulation in tea plants. However, less is known about the intricate network of Al³⁺ promoted F accumulation in tea plants. In this study, the involvement of endogenous Ca²⁺ and CaM in Al³⁺ pretreatment-promoted F accumulation in tea plants was investigated. Our results showed that Al³⁺ induced the inverse change of intracellular Ca²⁺ fluorescence intensity and stimulated Ca²⁺ trans-membrane transport in the mature zone of tea root. Also, a link between internal Ca²⁺ and CaM was found in tea roots under the presence of Al³⁺. In order to investigate whether Ca²⁺ and CaM were related to F accumulation promoted by Al³⁺ pretreatment, Ca²⁺ chelator EGTA and CaM antagonists CPZ and TFP were used. EGTA, CPZ, and TFP pretreatment inhibited Al³⁺-induced increase of Ca²⁺ fluorescence intensity and CaM content in tea roots, and also significantly reduced Al³⁺-promoted F accumulation in tea plants. Taken together, our results suggested that the endogenous Ca²⁺ and CaM are involved in Al³⁺ pretreatment-promoted F accumulation in tea roots.

Item Type:	Refereed Article
Keywords:	Al3+, calcium, calmodulin, fluoride, tea plant
Research Division:	Biological Sciences
Research Group:	Plant biology
Research Field:	Plant physiology
Objective Division:	Plant Production and Plant Primary Products
Objective Group:	Industrial crops
Objective Field:	Industrial crops not elsewhere classified
UTAS Author:	Wu, H-H (Mr Honghong Wu)
ID Code:	105559
Year Published:	2015
Web of Science® Times Cited:	25
Deposited By:	Tasmanian Institute of Agriculture
Deposited On:	2016-01-07
Last Modified:	2016-08-11
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