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Identification of the MAT1 locus in Stagonosporopsis tanaceti, and exploring its potential for sexual reproduction in Australian pyrethrum fields

Citation

Vaghefi, N and Ades, PK and Hay, FS and Pethybridge, SJ and Ford, R and Taylor, PWJ, Identification of the MAT1 locus in Stagonosporopsis tanaceti, and exploring its potential for sexual reproduction in Australian pyrethrum fields, Fungal Biology, 119, (5) pp. 408-419. ISSN 1878-6146 (2015) [Refereed Article]

Copyright Statement

Copyright 2014 The British Mycological Society

DOI: doi:10.1016/j.funbio.2014.04.004

Abstract

Stagonosporopsis chrysanthemi, S. inoxydabilis, and S. tanaceti are closely related Ascomycetes associated with ray blight of the Asteraceae. To date, only S. tanaceti has been identified in Australia, incurring substantial losses to the pyrethrum industry. In contrast to the homothallic S. chrysanthemi and S. inoxydabilis, a sexual state has not been observed for S. tanaceti. The MAT1 locus in S. tanaceti was identified through de novo assembly of shotgun reads, and was further used to develop primers for amplification of the full-length MAT1/2 locus in S. chrysanthemi and S. inoxydabilis. As expected, S. chrysanthemi and S. inoxydabilis possessed a MAT1/2 locus typical of homothallic Dothideomycetes with two adjacent MAT1-1 and MAT1-2 idiomorphs. However, only MAT1-1 could be detected in the assembled genome of S. tanaceti. Although a sexual mode of reproduction cannot be ruled out for S. tanaceti, evidence so far suggests this is absent or occurring at very low frequency in Australian pyrethrum fields.

Item Details

Item Type:Refereed Article
Keywords:Chrysanthemi, Didymellaceae, Inoxydabilis, mating type, ray blght
Research Division:Agricultural and Veterinary Sciences
Research Group:Crop and Pasture Production
Research Field:Crop and Pasture Protection (Pests, Diseases and Weeds)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Industrial Crops
Objective Field:Plant Extract Crops (e.g. Pyrethrum, Jojoba)
Author:Hay, FS (Dr Frank Hay)
ID Code:105555
Year Published:2015
Web of Science® Times Cited:6
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2016-01-07
Last Modified:2016-08-24
Downloads:0

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