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Destabilization of strigolactone receptor DWARF14 by binding of ligand and E3-ligase signaling effector DWARF3

Citation

Zhao, L-H and Zhou, XE and Yi, W and Wu, Z and Liu, Y and Kang, Y and Hou, L and de Waal, PW and Li, S and Jiang, Y and Scaffidi, A and Flemmati, GR and Smith, SM and Lam, VQ and Griffin, PR and Wang, Y and Li, J and Melcher, K and Xu, HE, Destabilization of strigolactone receptor DWARF14 by binding of ligand and E3-ligase signaling effector DWARF3, Cell Research, 25, (11) pp. 1219-1236. ISSN 1001-0602 (2015) [Refereed Article]

Copyright Statement

Copyright 2015 IBCB, SIBS, CAS

DOI: doi:10.1038/cr.2015.122

Abstract

Strigolactones (SLs) are endogenous hormones and exuded signaling molecules in plant responses to low levels of mineral nutrients. Key mediators of the SL signaling pathway in rice include the α/β-fold hydrolase DWARF 14 (D14) and the F-box component DWARF 3 (D3) of the ubiquitin ligase SCFD3 that mediate ligand-dependent degradation of downstream signaling repressors. One perplexing feature is that D14 not only functions as the SL receptor but is also an active enzyme that slowly hydrolyzes diverse natural and synthetic SLs including GR24, preventing the crystallization of a binary complex of D14 with an intact SL as well as the ternary D14/SL/D3 complex. Here we overcome these barriers to derive a structural model of D14 bound to intact GR24 and identify the interface that is required for GR24-mediated D14-D3 interaction. The mode of GR24-mediated signaling, including ligand recognition, hydrolysis by D14, and ligand-mediated D14-D3 interaction, is conserved in structurally diverse SLs. More importantly, D14 is destabilized upon the binding of ligands and D3, thus revealing an unusual mechanism of SL recognition and signaling, in which the hormone, the receptor, and the downstream effectors are systematically destabilized during the signal transduction process.

Item Details

Item Type:Refereed Article
Keywords:strigolactones, D14, D3, GR24, receptor/co-receptor
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Smith, SM (Professor Steven Smith)
ID Code:105513
Year Published:2015
Funding Support:Australian Research Council (DP140104567)
Web of Science® Times Cited:36
Deposited By:Plant Science
Deposited On:2016-01-06
Last Modified:2017-11-02
Downloads:0

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