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A Novel In Vitro Primary Culture Model of the Lower Motor Neuron–Neuromuscular Junction Circuit

Citation

Southam, KA and King, AE and Blizzard, CA and McCormack, GH and Dickson, T, A Novel In Vitro Primary Culture Model of the Lower Motor Neuron-Neuromuscular Junction Circuit, Microfluidic and Compartmentalized Platforms for Neurobiological Research, Springer, E Biffi (ed), United States, pp. 181-193. ISBN 978-1-4939-2509-4 (2015) [Other Book Chapter]

Copyright Statement

Copyright 2015 Springer Science+Business Media New York

DOI: doi:10.1007/978-1-4939-2510-0_11

Abstract

Modelling the complex process of neuromuscular signalling is key to understanding not only normal circuit function but also importantly the mechanisms underpinning a range of degenerative diseases. Here, we describe a compartmented in vitro model of the lower motor neuron–neuromuscular junction circuit, incorporating primary spinal motor neurons, supporting glia and skeletal muscle. This culture model is designed to spatially mimic the unique anatomical and cellular interactions of this circuit in compartmented microfluidic devices, such that the glial cells are located with motor neuron cell bodies in the cell body chamber and motor neuron axons extend to a distal chamber containing skeletal muscle cells whilst simultaneously allowing targeted intervention.

Item Details

Item Type:Other Book Chapter
Keywords:Neuromuscular junction, microfluidic, motor neuron
Research Division:Medical and Health Sciences
Research Group:Neurosciences
Research Field:Cellular Nervous System
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Nervous System and Disorders
Author:Southam, KA (Dr Katherine Southam)
Author:King, AE (Associate Professor Anna King)
Author:Blizzard, CA (Dr Catherine Blizzard)
Author:McCormack, GH (Mr Graeme McCormack)
Author:Dickson, T (Associate Professor Tracey Dickson)
ID Code:105380
Year Published:2015
Deposited By:Wicking Dementia Research and Education Centre
Deposited On:2015-12-22
Last Modified:2016-08-10
Downloads:0

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