eCite Digital Repository

A Novel In Vitro Primary Culture Model of the Lower Motor Neuron-Neuromuscular Junction Circuit


Southam, KA and King, AE and Blizzard, CA and McCormack, GH and Dickson, T, A Novel In Vitro Primary Culture Model of the Lower Motor Neuron-Neuromuscular Junction Circuit, Microfluidic and Compartmentalized Platforms for Neurobiological Research, Springer, E Biffi (ed), United States, pp. 181-193. ISBN 978-1-4939-2509-4 (2015) [Other Book Chapter]

Copyright Statement

Copyright 2015 Springer Science+Business Media New York

DOI: doi:10.1007/978-1-4939-2510-0_11


Modelling the complex process of neuromuscular signalling is key to understanding not only normal circuit function but also importantly the mechanisms underpinning a range of degenerative diseases. Here, we describe a compartmented in vitro model of the lower motor neuron–neuromuscular junction circuit, incorporating primary spinal motor neurons, supporting glia and skeletal muscle. This culture model is designed to spatially mimic the unique anatomical and cellular interactions of this circuit in compartmented microfluidic devices, such that the glial cells are located with motor neuron cell bodies in the cell body chamber and motor neuron axons extend to a distal chamber containing skeletal muscle cells whilst simultaneously allowing targeted intervention.

Item Details

Item Type:Other Book Chapter
Keywords:Neuromuscular junction, microfluidic, motor neuron
Research Division:Biomedical and Clinical Sciences
Research Group:Neurosciences
Research Field:Cellular nervous system
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Southam, KA (Dr Katherine Southam)
UTAS Author:King, AE (Professor Anna King)
UTAS Author:Blizzard, CA (Dr Catherine Blizzard)
UTAS Author:McCormack, GH (Mr Graeme McCormack)
UTAS Author:Dickson, T (Professor Tracey Dickson)
ID Code:105380
Year Published:2015
Deposited By:Wicking Dementia Research and Education Centre
Deposited On:2015-12-22
Last Modified:2018-05-07

Repository Staff Only: item control page