Shukla, SD and Muller, HK and Latham, R and Sohal, SS and Walters, EH, Platelet-activating factor receptor (PAFr) is upregulated in small airways and alveoli of smokers and COPD patients, Respirology, 21, (3) Article 12709. ISSN 1323-7799 (2016) [Refereed Article]
Copyright 2015 Asian Pacific Society of Respirology
Background and objective: PAFr is a cell adhesion site for specific bacteria, notably non-typeable Haemophilus influenzae (NTHi) and Streptococcus pneumoniae. We previously published that PAFr expression is significantly upregulated in the large airways of smokers, especially in COPD. We have now investigated PAFr expression in the epithelium and Rbm of small airways and in the alveolar compartment in smokers and patients with both COPD and small airway disease.
Methods: We evaluated PAFr expression crosssectionally in resected lung tissue from: eight smokers with normal lung function (NLFS); 10 with smokingrelated small airway narrowing only; eight COPD smokers; 10 COPD ex-smokers, and compared these with nine control tissues. Anti-PAFr immunostaining was quantified using computer-aided image analysis.
Results: Significantly increased PAFr expression in small airway epithelium of all clinical groups was found compared with controls (P < 0.01). Moreover, epithelial PAFr expression was upregulated in COPD smokers compared with NLFS (P < 0.05), but not when compared with COPD ex-smokers or patients with only small airways disease. Smoking history (pack-year) correlated significantly with PAFr expression in the currently smoking individuals, especially in NLFS (r = 0.9; P < 0.002). An increase above normal in PAFr-expressing cells in the airway epithelial Rbm was only significant in COPD smokers (P < 0.007). An upregulation of PAFr-expressing cell in alveolar epithelium was uniformly found in all clinical groups compared with normal control (P < 0.01).
|Item Type:||Refereed Article|
|Keywords:||platelet-activating factor, COPD, smoking, airway epithelium, chronic obstructive pulmonary disease, inhaled corticosteroid, platelet-activating factor receptor, respiratory bacteria adhesion|
|Research Division:||Medical and Health Sciences|
|Research Group:||Cardiorespiratory Medicine and Haematology|
|Research Field:||Respiratory Diseases|
|Objective Group:||Clinical Health (Organs, Diseases and Abnormal Conditions)|
|Objective Field:||Respiratory System and Diseases (incl. Asthma)|
|UTAS Author:||Shukla, SD (Mr Shakti Shukla)|
|UTAS Author:||Muller, HK (Professor Konrad Muller)|
|UTAS Author:||Latham, R (Mr Roger Latham)|
|UTAS Author:||Sohal, SS (Dr Sukhwinder Sohal)|
|UTAS Author:||Walters, EH (Professor Haydn Walters)|
|Funding Support:||National Health and Medical Research Council (1020515)|
|Web of Science® Times Cited:||21|
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