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Ion flux kinetics in blue light-grown field dodder (Cuscuta campestris) seedlings

Citation

Haidar, M and Shabala, S, Ion flux kinetics in blue light-grown field dodder (Cuscuta campestris) seedlings, Weed Biology and Management, 15, (4) pp. 159-164. ISSN 1444-6162 (2015) [Refereed Article]

Copyright Statement

Copyright 2015 Weed Science Society of Japan

DOI: doi:10.1111/wbm.12079

Abstract

Previous studies have revealed that blue light stimulates coiling and prehaustoria development in young de-etiolated dodder seedlings prior to host attachment. In this study, the Ca2+ and H+ flux kinetics that are associated with blue light-mediated coiling and prehaustoria development in de-etiolated dodder seedlings were measured uninvasively using the ion-selective, vibrating microelectrode ion flux system. The findings showed that blue light induces rapid transient changes in the net Ca2+ and H+ fluxes. A clearly pronounced lag of about several minutes was observed between the blue light-induced changes in the Ca2+ and H+ fluxes, with H+ being a leading ion. The addition of 1 mol L−1 vanadate, a known blocker of the plasma-membrane H+-ATPase, to the bathing solution completely prevented both the H+ and the Ca2+ flux responses and inhibited coiling and prehaustoria development, suggesting a causal relationship between the blue light-induced changes in the net Ca2+ and H+ fluxes and seedling movements. It is concluded that the plasma-membrane H+-ATPase plays a key role in a dodder's parasitic mode of growth and its adaptive response to the environment.

Item Details

Item Type:Refereed Article
Keywords:weed, light, signalling, blue light, dodder, H+-ATPase, ion fluxes
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Shabala, S (Professor Sergey Shabala)
ID Code:105130
Year Published:2015
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2015-12-09
Last Modified:2017-11-06
Downloads:0

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