Modulation of triglyceride accumulation in adipocytes by psychopharmacological agents in vitro
Loffler, D and Landgraf, K and Korner, A and Kratzsch, J and Kirkby, KC and Himmerich, E, Modulation of triglyceride accumulation in adipocytes by psychopharmacological agents in vitro, Journal of Psychiatric Research, 72 pp. 37-42. ISSN 0022-3956 (2016) [Refereed Article]
Weight gain is a major problem during psychopharmacological treatment. Research has concentrated on
the appetite inducing properties and mechanisms of these drugs in the central nervous system. The
potential contribution of direct effects of drugs on metabolically relevant peripheral cells such as adipocytes
is less well understood.
We examined the influence of the antidepressant imipramine, the antipsychotic clozapine, and the
mood stabilizer lithium on preadipocytes and adipocytes in vitro, using Simpson-Golabi-Behmel syndrome
(SGBS) cells, an established human preadipocyte model. Parameters of cell differentiation and
signaling, and cell metabolism were measured.
We found significantly increased triglyceride accumulation in adipocytes after supplementation with
imipramine and lithium at therapeutic concentrations, compared to non-supplemented control samples.
However, gene expression levels of an early marker of adipogenesis, the peroxisome proliferatoractivated
receptor gamma (PPAR-g) and a late marker of adipogenesis, the fatty acid binding protein 4
(FABP4), as well as expression of adiponectin (ADIPOQ) did not change significantly in the presence of
these psychopharmacological agents.
The results suggest a direct influence of imipramine and lithium but not clozapine on fat storage of
adipocytes. The underlying mechanisms of fatty acid storage and adipocyte differentiation however
remain to be elucidated.