eCite Digital Repository

Fingerprinting and pedigree analysis in Eucalyptus globulus using RAPDs


Nesbitt, KA and Potts, BM and Vaillancourt, RE and Reid, JB, Fingerprinting and pedigree analysis in Eucalyptus globulus using RAPDs, Silvae Genetica, 46, (1) pp. 6-11. ISSN 0037-5349 (1997) [Refereed Article]


RAPDs were used to confirm clonal fidelity and distinguish individuals of varying degrees of relationship in Eucalyptus globulus. RAPD variation was examined amongst ramets derived from 10 ortets which were either full-sibs, half-sibs, or unrelated, as well as their parents. Genetic similarity amongst samples was calculated from 117 RAPD hands using the simple matching coefficient. RAPD variation within clones was trivial compared to the variation found even between full-sib chines. Genetic similarity decreased with pedigree distance. The pedigree of the material was successfully displayed by UPGMA clustering of the genetic similarity matrix, with parents generally clustering with their offspring. In a second experiment, 120 RAPD hands allowed the successful discrimination of selfs from their intra- and interprovenance half-sib outcrosses and again genetic similarity decreased with pedigree distance. These results clearly indicate that RAPDs have great potential for estimation of outcrossing rates and pedigree analysis.

Item Details

Item Type:Refereed Article
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Forestry sciences
Research Field:Forestry management and environment
Objective Division:Plant Production and Plant Primary Products
Objective Group:Forestry
Objective Field:Hardwood plantations
UTAS Author:Nesbitt, KA (Dr Katherine Nesbitt)
UTAS Author:Potts, BM (Professor Brad Potts)
UTAS Author:Vaillancourt, RE (Professor Rene Vaillancourt)
UTAS Author:Reid, JB (Professor Jim Reid)
ID Code:10460
Year Published:1997
Web of Science® Times Cited:17
Deposited By:Plant Science
Deposited On:1997-08-01
Last Modified:2010-11-08

Repository Staff Only: item control page