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The association of different urinary proteins with calcium oxalate hydromorphs. Evidence for non-specific interactions

Citation

Walton, RC and Kavanagh, JP and Heywood, BR and Rao, PN, The association of different urinary proteins with calcium oxalate hydromorphs. Evidence for non-specific interactions, Biochimica et Biophysica Acta - General Subjects, 1723, (1-3) pp. 175-183. ISSN 0304-4165 (2005) [Refereed Article]

DOI: doi:10.1016/j.bbagen.2005.02.014

Abstract

It has been proposed that various urinary proteins interact specifically with different calcium oxalate hydromorphs and these interactions have important implications regarding the understanding of the onset and progress of kidney stone disease. Calcium oxalate monohydrate and dihydrate crystals were grown and characterised thoroughly to establish sample purity. These crystals were then incubated in artificial urine samples containing isolated urinary macromolecules. Crystal growth was prevented by saturating the incubation mix with calcium oxalate, and this was confirmed through electron microscopy and calcium measurements of the incubation mix. The surface interactions between the different calcium oxalate hydrates and urinary proteins were investigated by the use of Western blots and immunoassays. The same proteins, notably albumin, Tamm-Horsfall protein, osteopontin and prothrombin fragment 1, associated with both hydrates. There was a trend for more protein to associate with calcium oxalate dihydrate, and greater quantities of different proteins associated with both hydrates when Tamm-Horsfall protein was removed from the incubation mix. There is no evidence from this study to indicate that particular proteins interact with specific calcium oxalate hydrates, which in turn suggests that these protein-mineral interactions are likely to be mediated through non-specific charge interactions.

Item Details

Item Type:Refereed Article
Keywords:biomineralisation, calcium oxalate, crystal matrix, protein matrix, urolithiasis
Research Division:Chemical Sciences
Research Group:Physical Chemistry (incl. Structural)
Research Field:Structural Chemistry and Spectroscopy
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Heywood, BR (Professor Brigid Heywood)
ID Code:104472
Year Published:2005
Web of Science® Times Cited:21
Deposited By:Research Division
Deposited On:2015-11-12
Last Modified:2015-11-12
Downloads:0

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