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Electron microscopy study of magnetosomes in a cultured coccoid magnetotactic bacterium

Citation

Meldrum, FC and Mann, S and Heywood, BR and Frankel, RB and Bazylinski, DA, Electron microscopy study of magnetosomes in a cultured coccoid magnetotactic bacterium, Proceedings of the Royal Society B: Biological Sciences, 251, (1332) pp. 231-236. ISSN 0962-8452 (1993) [Refereed Article]

Copyright Statement

Copyright 1993 The Royal Society

DOI: doi:10.1098/rspb.1993.0034

Abstract

Intracellular magnetite (Fe3O4) crystals produced by the magnetotactic bacterium MC-1 were analysed by transmission electron microscopy (TEM). Strain MC-1 represents the first-reported isolation of a coccoid magnetotactic bacterium in axenic culture. The magnetosomes of this bacterium displayed a pseudo-hexagonal prismatic habit, were elongated along the <111> crystallographic direction, and were truncated by {111}, {100} and {110} faces. The presence of {111} truncations represents a modification of the magnetosome morphology previously determined for those in other coccoid bacteria. Study of immature crystals produced by strain MC-1 showed that the crystal morphology was controlled even at early stages of development. Changes in the culture media affected both the number and shape of the bacterial magnetite crystals. Cells grown in an acetate-containing medium contained on average more crystals than those in cells grown in a sulphide-containing medium. Crystals synthesized in the acetate-grown cells tended to be less truncated than those in the sulphide-grown cells. No iron sulphide minerals, such as greigite, were observed in cells grown in the presence of sulphide.

Item Details

Item Type:Refereed Article
Keywords:acetic acid, magnetite, bacteria, Coccoidea, Negibacteria
Research Division:Biological Sciences
Research Group:Biochemistry and Cell Biology
Research Field:Structural Biology (incl. Macromolecular Modelling)
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Heywood, BR (Professor Brigid Heywood)
ID Code:104425
Year Published:1993
Web of Science® Times Cited:115
Deposited By:Research Division
Deposited On:2015-11-11
Last Modified:2015-12-21
Downloads:0

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