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Extraction of calcium containing crystals from synovial fluids and articular cartilage


Swan, AJ and Heywood, BR and Dieppe, PA, Extraction of calcium containing crystals from synovial fluids and articular cartilage, Journal of Rheumatology, 19, (11) pp. 1765-1773. ISSN 0315-162X (1992) [Refereed Article]


A protocol for extracting minerals from biological tissues and fluids has been developed. Synovial fluid (SF) and articular cartilage samples were treated with papain and sodium hypochlorite to extract and concentrate calcium containing crystals. The method validated by testing the ability to reextract synthetic calcium pyrophosphate dihydrate and calcium hydroxyapatite crystals added to SF in an unchanged form. The extracted crystals were analyzed by infrared spectroscopy and X-ray powder diffraction. The concentration of both calcium pyrophosphate dihydrate and hydroxyapaptite crystals in SF was found to be higher than published figures.

Item Details

Item Type:Refereed Article
Keywords:calcium pyrophosphate, cartilage, crystals, hydroxyapatite, synovial fluid
Research Division:Chemical Sciences
Research Group:Macromolecular and materials chemistry
Research Field:Structure and dynamics of materials
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Heywood, BR (Professor Brigid Heywood)
ID Code:104418
Year Published:1992
Deposited By:Research Division
Deposited On:2015-11-11
Last Modified:2015-11-12

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