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Magnetoferritin: In vitro synthesis of a novel magnetic protein

Citation

Meldrum, FC and Heywood, BR and Mann, S, Magnetoferritin: In vitro synthesis of a novel magnetic protein, Science, 257, (5069) pp. 522-523. ISSN 0036-8075 (1992) [Refereed Article]

Copyright Statement

Copyright 1992 The Authors

DOI: doi:10.1126/science.1636086

Abstract

The iron storage protein ferritin consists of a spherical polypeptide shell (apoferritin) surrounding a 6-nanometer inorganic core of the hydrated iron oxide ferrihydrite (5Fe2O39H2O). Previous studies have shown that the in vitro reconstitution of apoferritin yields mineral cores essentially identical to those of the native proteins. A magnetic mineral was synthesized within the nanodimensional cavity of horse spleen ferritin by the use of controlled reconstitution conditions. Transmission electron microscopy and electron diffraction analysis indicate that the entrapped mineral particles are discrete 6-nanometer spherical single crystals of the ferrimagnetic iron oxide magnetite (Fe3O4). The resulting magnetic protein, "magnetoferritin," could have uses in biomedical imaging, cell labeling, and separation procedures.

Item Details

Item Type:Refereed Article
Keywords:ferric ion, ferric oxide, ferritin, horses, magnetics, microscopy, electron, protein conformation, spleen
Research Division:Biological Sciences
Research Group:Biochemistry and Cell Biology
Research Field:Structural Biology (incl. Macromolecular Modelling)
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Heywood, BR (Professor Brigid Heywood)
ID Code:104417
Year Published:1992
Web of Science® Times Cited:450
Deposited By:Research Division
Deposited On:2015-11-11
Last Modified:2015-12-21
Downloads:0

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