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Counter-pressure-assisted ITP with electrokinetic injection under field-amplified conditions for bacterial analysis

Citation

Phung, SC and Nai, YH and Macka, M and Powell, SM and Guijt, RM and Breadmore, MC, Counter-pressure-assisted ITP with electrokinetic injection under field-amplified conditions for bacterial analysis, Analytical and Bioanalytical Chemistry, 407, (23) pp. 6995-7002. ISSN 1618-2642 (2015) [Refereed Article]

Copyright Statement

Copyright 2015 Springer-Verlag Berlin Heidelberg

DOI: doi:10.1007/s00216-015-8838-4

Abstract

Counter-pressure was used to extend the duration of field-amplified sample injection in isotachophoresis (FASI-ITP) in order to improve the detection of bacterial cells. Using 0.51-μm negatively charged encapsulated fluorescent beads as a model, the counter-pressure, injection and separation voltages, and times were optimized. Using 6-min 8,963-Pa counter-pressure FASI-ITP injections at −12 kV followed by mobilization of the ITP band with continued injection at −6 kV, the limit of detection (LOD) for Escherichia coli was improved to 78 cells/mL, a factor of 4 when compared with FASI-ITP without counter-pressure.

Item Details

Item Type:Refereed Article
Keywords:capillary isotachophoresis, bacterial quantification, capillary electrophoresis, cell systems, single cell analysis, counter-pressure, microbials, electrokinetic injections, field amplified
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Phung, SC (Ms Sui Phung)
Author:Macka, M (Professor Mirek Macka)
Author:Powell, SM (Dr Shane Powell)
Author:Guijt, RM (Dr Rosanne Guijt)
Author:Breadmore, MC (Professor Michael Breadmore)
ID Code:104310
Year Published:2015
Web of Science® Times Cited:5
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2015-11-09
Last Modified:2018-03-14
Downloads:0

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