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Shewanella gelidimarina sp. nov. and Shewanella frigidimarina sp. nov., novel Antarctic species with the ability to produce eicosapentaenoic acid (20:5 omega 3) and grow anaerobically by dissimilatory Fe(III) reduction

Citation

Bowman, JP and McCammon, SA and Nichols, DS and Skerratt, J and Rea, SM and Nichols, PD and McMeekin, TA, Shewanella gelidimarina sp. nov. and Shewanella frigidimarina sp. nov., novel Antarctic species with the ability to produce eicosapentaenoic acid (20:5 omega 3) and grow anaerobically by dissimilatory Fe(III) reduction, International Journal of Systematic Bacteriology, 47, (4) pp. 1040-1047. ISSN 0020-7713 (1997) [Refereed Article]

DOI: doi:10.1099/00207713-47-4-1040

Abstract

A polyphasic taxonomic study was performed to characterize dissimilatory iron-reducing strains mostly isolated from Antarctic sea ice. The strains were isolated from samples of congelated (land-fast) sea ice, grease ice, and ice algal biomass collected from the coastal areas of the Vestfold Hills in eastern Antarctica (68°S 78°E). The strains were facultatively anaerobic, motile, and rod shaped, were capable of anaerobic growth either by fermentation of carbohydrates or by anaerobic respiration, and utilized a variety of electron acceptors, including nitrate, ferric compounds, and trimethylamine N-oxide. A phylogenetic analysis performed with 16S rRNA sequences showed that the isolates formed two groups representing novel lineages in the genus Shewanella. The first novel group included seawater- requiring, psychrophilic, chitinolytic strains which had DNA G+C contents of 48 tool%. The members of the second strain group were psychrotrophic and did not require seawater but could tolerate up to 9% NaCI. The strains of this group were also unable to degrade polysaccharides but could utilize a number of monosaccharides and disaccharides and had G+C contents of 40 to 43 tool%. The whole-cell-derived fatty acid profiles of the sea ice isolates were found to be similar to the profiles obtained for other Shewanella species. The omega-3 polyunsaturated fatty acid eicosapentaenoic acid (EPA) (20:5ω3) was detected in all of the sea ice isolates at levels ranging from 2 to 16% of the total fatty acids. EPA was also found at high levels in Shewanella hanedai (19 to 22%) and Shewanella benthica (16 to 18%) but was absent in Shewanella alga and Shewanellla putrefaeiens. On the basis of polyphasic taxonomic data, the Antarctic iron-reducing strains are placed in two new species, Shewanella fridimarina sp. nov. (type strain, ACAM 591) and Shewanella gelidimarina sp. nov. (type strain, ACAM 456).

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Bacteriology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Environmental Sciences
Author:Bowman, JP (Associate Professor John Bowman)
Author:McCammon, SA (Ms Sharee McCammon)
Author:Nichols, DS (Dr David Nichols)
Author:Skerratt, J (Dr Jenny Skerratt)
Author:Rea, SM (Ms Suzanne May Rea)
Author:Nichols, PD (Dr Peter Nichols)
Author:McMeekin, TA (Professor Thomas McMeekin)
ID Code:10403
Year Published:1997
Web of Science® Times Cited:186
Deposited By:Agricultural Science
Deposited On:1997-08-01
Last Modified:2011-08-11
Downloads:0

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