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Calibration of a hand-held haemoglobin analyser for use on fish blood

Citation

Clark, TD and Eliason, EJ and Sandblom, E and Hinch, SG and Farrell, AP, Calibration of a hand-held haemoglobin analyser for use on fish blood, Journal of Fish Biology, 73, (10) pp. 2587-2595. ISSN 0022-1112 (2008) [Refereed Article]

DOI: doi:10.1111/j.1095-8649.2008.02109.x

Abstract

The HemoCue haemoglobin analyser consistently overestimated haemoglobin concentration ([Hb]) in the blood of all fish species (sockeye salmon Oncorhynchus nerka, Chinook salmon Oncorhynchus tshawytscha, Pacific bluefin tuna Thunnus orientalis and chub mackerel Scomber japonicus) by 22–50% (9·9–36·0 g l−1) over a [Hb] range of 20–160 g l−1. The systematic nature of the overestimation, however, allowed the formulation of an accurate calibration equation that can be used to correct values of [Hb] measured by the HemoCue in field studies.

Item Details

Item Type:Refereed Article
Keywords:Drabkin’s method, haemoglobin concentration, haemoglobinometer, HemoCue Hb 201+, spectrophotometer
Research Division:Biological Sciences
Research Group:Zoology
Research Field:Animal Behaviour
Objective Division:Environment
Objective Group:Flora, Fauna and Biodiversity
Objective Field:Coastal and Estuarine Flora, Fauna and Biodiversity
UTAS Author:Clark, TD (Dr Timothy Clark)
ID Code:103461
Year Published:2008
Web of Science® Times Cited:45
Deposited By:IMAS Research and Education Centre
Deposited On:2015-10-12
Last Modified:2015-10-12
Downloads:0

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