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Alginate microencapsulation for oral immunisation of finfish: release characteristics, ex vivo intestinal uptake and in vivo administration in Atlantic salmon, Salmo salar L

Citation

Ghosh, B and Nowak, BF and Bridle, AR, Alginate microencapsulation for oral immunisation of finfish: release characteristics, ex vivo intestinal uptake and in vivo administration in Atlantic salmon, Salmo salar L, Marine Biotechnology, 17, (6) pp. 841-853. ISSN 1436-2228 (2015) [Refereed Article]

Copyright Statement

Copyright Springer Science+Business Media New York 2015

DOI: doi:10.1007/s10126-015-9663-7

Abstract

This study examined the feasibility of alginate microcapsules manufactured using a low-impact technology and reagents to protect orally delivered immunogens for use as immunoprophylactics for fish. Physical characteristics and protein release kinetics of the microcapsules were examined at different pH and temperature levels using a microencapsulated model protein, bovine serum albumin (BSA). Impact of the microencapsulation process on contents was determined by analysing change in bioactivity of microencapsulated lysozyme. Feasibility of the method for oral immunoprophylaxis of finfish was assessed using FITC-labelled microcapsules. These were applied to distal intestinal explants of Atlantic salmon (Salmo salar) to investigate uptake ex vivo. Systemic distribution of microcapsules was investigated by oral administration of FITC-labelled microcapsules to Atlantic salmon fry by incorporating into feed. The microcapsules produced were structurally robust and retained surface integrity, with a modal size distribution of 250–750 nm and a tendency to aggregate. Entrapment efficiency of microencapsulation was 51.2 % for BSA and 43.2 % in the case of lysozyme. Microcapsules demonstrated controlled release of protein, which increased with increasing pH or temperature, and the process had no significant negative effect on bioactivity of lysozyme. Uptake of fluorescent-labelled microcapsules was clearly demonstrated by intestinal explants over a 24-h period. Evidence of microcapsules was found in the intestine, spleen, kidney and liver of fry following oral administration. Amenability of the microcapsules to intestinal uptake and distribution reinforced the strong potential for use of this microencapsulation method in oral immunoprophylaxis of finfish using sensitive immunogenic substances.

Item Details

Item Type:Refereed Article
Keywords:oral vaccination, Atlantic salmon, microencapsulation
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Fisheries - Aquaculture not elsewhere classified
Author:Ghosh, B (Dr Bikramjit Ghosh)
Author:Nowak, BF (Professor Barbara Nowak)
Author:Bridle, AR (Dr Andrew Bridle)
ID Code:103224
Year Published:2015
Deposited By:IMAS Research and Education Centre
Deposited On:2015-09-29
Last Modified:2017-11-04
Downloads:0

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