File(s) under permanent embargo
Physicochemical quantification of abscisic acid levels in plant tissues with an added internal standard by ultra-performance liquid chromatography
journal contribution
posted on 2023-05-22, 02:35 authored by McAdam, SAMThe phytohormone abscisic acid (ABA) is critical for a range of plant responses to the environment, most importantly in closing the stomata of seed plants during drought (Mittelheuser and Van Steveninck 1969; Brodribb et al. 2014). The high precision quantification of this hormone by physicochemical methods is a relatively simple process, essential for studies that aim to investigate the role or action of this hormone in plants. Outlined here is a method for the extraction, purification and quantification of ABA levels in plant tissues. This method involves methanolic extraction of ABA from homogenised tissue. Purification of ABA is then undertaken by a simple etheric partitioning method. A focus is placed on determining ABA levels in leaves; however this method is suitable for all tissue types, including plant solutes such as xylem sap, seeds (both dry and green) and large samples of tissue such as root systems.
Funding
Australian Research Council
History
Publication title
Bio-ProtocolVolume
5Issue
18Article number
e1599Number
e1599Pagination
1-13ISSN
2331-8325Department/School
School of Natural SciencesPublisher
Bio-protocolPlace of publication
United States of AmericaRights statement
Copyright 2015 The AuthorRepository Status
- Restricted