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Physicochemical quantification of abscisic acid levels in plant tissues with an added internal standard by ultra-performance liquid chromatography

journal contribution
posted on 2023-05-22, 02:35 authored by McAdam, SAM
The phytohormone abscisic acid (ABA) is critical for a range of plant responses to the environment, most importantly in closing the stomata of seed plants during drought (Mittelheuser and Van Steveninck 1969; Brodribb et al. 2014). The high precision quantification of this hormone by physicochemical methods is a relatively simple process, essential for studies that aim to investigate the role or action of this hormone in plants. Outlined here is a method for the extraction, purification and quantification of ABA levels in plant tissues. This method involves methanolic extraction of ABA from homogenised tissue. Purification of ABA is then undertaken by a simple etheric partitioning method. A focus is placed on determining ABA levels in leaves; however this method is suitable for all tissue types, including plant solutes such as xylem sap, seeds (both dry and green) and large samples of tissue such as root systems.

Funding

Australian Research Council

History

Publication title

Bio-Protocol

Volume

5

Issue

18

Article number

e1599

Number

e1599

Pagination

1-13

ISSN

2331-8325

Department/School

School of Natural Sciences

Publisher

Bio-protocol

Place of publication

United States of America

Rights statement

Copyright 2015 The Author

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the biological sciences

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    University Of Tasmania

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