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Physicochemical quantification of abscisic acid levels in plant tissues with an added internal standard by ultra-performance liquid chromatography

Citation

McAdam, SAM, Physicochemical quantification of abscisic acid levels in plant tissues with an added internal standard by ultra-performance liquid chromatography, Bio-Protocol, 5, (18) Article e1599. ISSN 2331-8325 (2015) [Contribution to Refereed Journal]

Copyright Statement

Copyright 2015 The Author

Official URL: http://www.bio-protocol.org/e1599

Abstract

The phytohormone abscisic acid (ABA) is critical for a range of plant responses to the environment, most importantly in closing the stomata of seed plants during drought (Mittelheuser and Van Steveninck 1969; Brodribb et al. 2014). The high precision quantification of this hormone by physicochemical methods is a relatively simple process, essential for studies that aim to investigate the role or action of this hormone in plants. Outlined here is a method for the extraction, purification and quantification of ABA levels in plant tissues. This method involves methanolic extraction of ABA from homogenised tissue. Purification of ABA is then undertaken by a simple etheric partitioning method. A focus is placed on determining ABA levels in leaves; however this method is suitable for all tissue types, including plant solutes such as xylem sap, seeds (both dry and green) and large samples of tissue such as root systems.

Item Details

Item Type:Contribution to Refereed Journal
Keywords:quantification of abscisic acid
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
UTAS Author:McAdam, SAM (Dr Scott McAdam)
ID Code:103143
Year Published:2015
Funding Support:Australian Research Council (DE140100946)
Deposited By:Plant Science
Deposited On:2015-09-22
Last Modified:2016-08-02
Downloads:0

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