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Improvements to the RTgill-W1 fish gill assay for ichthyotoxins: a comparison of the potency of different toxin fractions and extracts tested with different microplate materials

Citation

Dorantes-Aranda, JJ and Seger, A and Mardones, JI and Place, AR and Hallegraeff, GM, Improvements to the RTgill-W1 fish gill assay for ichthyotoxins: a comparison of the potency of different toxin fractions and extracts tested with different microplate materials, Marine and Fresh-water Harmful Algae: Proceedings of the 16th International Conference on Harmful Algae, 27-31 October 2014, Wellington, New Zealand, pp. 202-205. ISBN 978-87-990827-5-9 (2015) [Refereed Conference Paper]


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Abstract

Different microplate materials were compared for in vitro screening of purified phycotoxins and algal extracts using the fish gill cell line RTgill-Wl. Glass plates coated with collagen I were more suitable for assays with brevetoxin and for methanol extracts of the haptophyte Prymnesium parvum (up to 33% more toxic). By contrast, polystyrene plates showed better results with karlotoxin (19% more toxic), and with extracts from the dinoflagellates Karlodinium veneficum, Karenia mikimotoi and Amphidinium carterae (20- 37% more toxic). Care should also be taken to conduct tests in light or dark conditions when toxins or extracts are light sensitive, as was found with Chattonella, Fibrocapsa and Prymnesium extracts (53-90% difference among results). As a first screening step for unknown toxins the use of both glass and plastic plates is strongly recommended. The differential behaviour of ichthyotoxins in plastic or glass containers and in light and dark conditions also contributes to our understanding on how these compounds can mediate fish kills in nature.

Item Details

Item Type:Refereed Conference Paper
Keywords:fish killing algal blooms, harmful phytoplankton, toxins, algal extracts, gill cells, RTgill-W1, ichthyotoxicity
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Phycology (incl. Marine Grasses)
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Fisheries - Aquaculture not elsewhere classified
Author:Dorantes-Aranda, JJ (Dr Juan Dorantes Aranda)
Author:Seger, A (Mr Andreas Seger)
Author:Mardones, JI (Mr Jorge Mardones)
Author:Hallegraeff, GM (Professor Gustaaf Hallegraeff)
ID Code:103058
Year Published:2015
Funding Support:Australian Research Council (DP130102859)
Deposited By:IMAS Research and Education Centre
Deposited On:2015-09-17
Last Modified:2016-04-21
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