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Improvements to the RTgill-W1 fish gill assay for ichthyotoxins: a comparison of the potency of different toxin fractions and extracts tested with different microplate materials
conference contribution
posted on 2023-05-23, 10:21 authored by Dorantes-Aranda, JJ, Andreas SegerAndreas Seger, Mardones, JI, Place, AR, Gustaaf HallegraeffGustaaf HallegraeffDifferent microplate materials were compared for in vitro screening of purified phycotoxins and algal extracts using the fish gill cell line RTgill-Wl. Glass plates coated with collagen I were more suitable for assays with brevetoxin and for methanol extracts of the haptophyte Prymnesium parvum (up to 33% more toxic). By contrast, polystyrene plates showed better results with karlotoxin (19% more toxic), and with extracts from the dinoflagellates Karlodinium veneficum, Karenia mikimotoi and Amphidinium carterae (20- 37% more toxic). Care should also be taken to conduct tests in light or dark conditions when toxins or extracts are light sensitive, as was found with Chattonella, Fibrocapsa and Prymnesium extracts (53-90% difference among results). As a first screening step for unknown toxins the use of both glass and plastic plates is strongly recommended. The differential behaviour of ichthyotoxins in plastic or glass containers and in light and dark conditions also contributes to our understanding on how these compounds can mediate fish kills in nature.
Funding
Australian Research Council
History
Publication title
Marine and Fresh-water Harmful Algae: Proceedings of the 16th International Conference on Harmful AlgaeEditors
A Lincoln MacKenziePagination
202-205ISBN
978-87-990827-5-9Department/School
Institute for Marine and Antarctic StudiesPublisher
Nelson, New ZealandPlace of publication
Cawthron InstituteEvent title
16th International Conference on Harmful AlgaeEvent Venue
Wellington, New ZealandDate of Event (Start Date)
2014-10-27Date of Event (End Date)
2014-10-31Rights statement
Copyright unknownRepository Status
- Restricted