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Molecular characterization of a mutation affecting abscisic acid biosynthesis and consequently stomatal responses to humidity in an agriculturally important species
Citation
McAdam, SAM and Sussmilch, FC and Brodribb, TJ and Ross, JJ, Molecular characterization of a mutation affecting abscisic acid biosynthesis and consequently stomatal responses to humidity in an agriculturally important species, AoB Plants, 7 Article plv091. ISSN 2041-2851 (2015) [Refereed Article]
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Copyright Statement
Copyright 2015 The Authors Licenced under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/)
DOI: doi:10.1093/aobpla/plv091
Abstract
Mutants deficient in the phytohormone abscisic acid (ABA) have been instrumental in determining not only the biosynthetic
pathway for this hormone, but also its physiological role in land plants. The wilty mutant of Pisum sativum is one of the classic, well-studied ABA deficient mutants; however this mutant remains uncharacterised at a molecular level.
Using a candidate gene approach, we show that the wilty mutation affects the xanthoxin dehydrogenase step in ABA biosynthesis. To date, this step has only been represented by mutants
in the ABA2 gene of Arabidopsis thaliana. Functional ABA biosynthesis appears to be critical for normal stomatal responses to changes in humidity in angiosperms,
with wilty mutant plants having no increase in foliar ABA levels in response to a doubling in vapour pressure deficit and no closure
of stomata. Phylogenetic analysis of the ABA2 gene family from diverse land plants indicates that the evolution of an ABA-biosynthetic-specific short-chain dehydrogenase
(ABA2) evolved in the earliest angiosperms. The relatively recent evolutionary origin of specificity in this step has important
implications for both the evolution of ABA biosynthesis and action in land plants.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | abscisic acid (ABA), biosynthesis, evolution, humidity, Pisum sativum, stomata, vapour pressure deficit, wilty mutant |
| Research Division: | Biological Sciences |
| Research Group: | Plant biology |
| Research Field: | Plant physiology |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding knowledge |
| Objective Field: | Expanding knowledge in the biological sciences |
| UTAS Author: | McAdam, SAM (Dr Scott McAdam) |
| UTAS Author: | Sussmilch, FC (Dr Frances Sussmilch) |
| UTAS Author: | Brodribb, TJ (Professor Tim Brodribb) |
| UTAS Author: | Ross, JJ (Associate Professor John Ross) |
| ID Code: | 102992 |
| Year Published: | 2015 |
| Funding Support: | Australian Research Council (DE140100946) |
| Web of Science® Times Cited: | 25 |
| Deposited By: | Plant Science |
| Deposited On: | 2015-09-15 |
| Last Modified: | 2017-11-01 |
| Downloads: | 640 View Download Statistics |
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